Atefeh Kamallou; Mahbobeh Haji Abdolbaghi; Minoo Mohraz; Mernaz Rasolinejad; Ehsan Karbasi; Bita Ansaripour; Samaneh Soltani; Arezou Rezaei; Neda Khalili; Aliakbar Amirzargar
Volume 11, Issue 4 , December 2014, , Pages 221-232
Abstract
Background: Lymphocyte subsets enumeration is considered prominent in the management of primary and acquired immunodeficiency disorders. Because of local variations due to race, age, gender, and environmental conditions on lymphocyte subsets, and to improve the accuracy of interpretation of laboratory ...
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Background: Lymphocyte subsets enumeration is considered prominent in the management of primary and acquired immunodeficiency disorders. Because of local variations due to race, age, gender, and environmental conditions on lymphocyte subsets, and to improve the accuracy of interpretation of laboratory findings, reference intervals must be determined in every population. Objective: To establish a normal reference range for CD3+ , CD4+ , CD8+ , CD19+ and CD56+ lymphocytes in a healthy Iranian adult population using flowcytometry. Method: Blood samples were collected from 221 HIV seronegative individuals, including 112 females and 109 males, with ages ranging from 20 to 40 years old. The percentage of lymphocytes expressing either of CD3, CD4, CD8, CD19 and CD56 surface markers were determined by flowcytometry assay. Result: Total mean percentage and absolute count of lymphocyte subsets were as follows: CD3+ : 70.90 ± 7.54%, 1800.87 ± 471.09 cells/µl; CD4+ : 41.04 ± 7.86%, 1039.99 ± 338.02 cells/µl; CD8+ : 31.11 ± 6.60%, 783.95 ± 234.87 cells/µl; CD19+ : 12.77 ± 4.56%, 328.37 ± 153.17 cells/µl; CD56+ : 15.53 ± 6.34%, 388.62 ± 176.17 cells/µl, respectively. The ratio of CD4+ /CD8+ lymphocytes for the studied population was 1.39 ± 0.48. Significant differences were observed between male and female subjects indicating that the average percentage of CD3+ cells (p=0.017) and CD4+ T cells (p=0.003) were higher in the female population, whereas the average percentage of CD19+ cells (p=0.02) tended to be higher among males. However, investigations on the CD56+ NK cell and CD8+ T cell sub-populations did not show any statistical differences between the two genders. In comparison with reports of other populations, we were confronted with different results. Conclusion: Establishing reference values of lymphocyte subsets for each population is helpful in achieving standard criteria for the prognosis of HIV infection. Therefore, normal ranges established by this survey can be used as a reference for decisions made in clinical practice.
Sayeed Bayanolhagh; Mahtab Alinezhad; Kooroosh Kamali; Maryam Foroughi; Hamid Reza Khorram Khorshid; Minoo Mohraz; Fereidoun Mahboudi; Ali Akbar Pourfathollah
Volume 7, Issue 3 , September 2010, , Pages 162-176
Abstract
Background: Numerous evidences indicate that in some HIV-1 positive patients, the humoral and cellular immune responses are induced against HIV-1 proteins and this is inversely related to the progress of infection. Objective: The aim of this study was the evaluation of the Adenovectors containing HIV ...
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Background: Numerous evidences indicate that in some HIV-1 positive patients, the humoral and cellular immune responses are induced against HIV-1 proteins and this is inversely related to the progress of infection. Objective: The aim of this study was the evaluation of the Adenovectors containing HIV genes in induction of immune responses in mice. Methods: The HIV-1 genes including gag p24, rev, nef and exon-1 of tat were amplified from HIV-1 RNA (clade-A). The cDNA of each gene was cloned into a transfer vector. The transfer vector was then co-transformed into E. coli strain BJ5183 together with pAdenovector ΔE1/E3. The recombinant adenoviral construct was transfected into QBI-293A cells. Recombinant viruses were purified and titrated on 293 cell plates. Expression of transgenes was evaluated using western blotting. Then 1012 viral particles were injected into 15 groups of 5 mice and all patterns of combination of these 4 HIV-1 genes were evaluated. After 2 weeks, humoral and cellular immune responses were evaluated using ELISA, cell proliferation and ELISpot (IL-2, IL-4 and IFN-γ) assays, consecutively. Results: It was demonstrated that each gene was expressed. The response targets were mostly toward Th1, though several Th2 responses were also observed. Single injection in our study induced a good cellular response but the humoral responses were not as strong as the cellular ones. Conclusion: Considering and comparing all results and evaluating the various possible interactions revealed that simultaneous injection of tat and gag has enhanced the humoral and cellular responses.