Parisa Yavari Kia; Behzad Baradaran; Mahnaz Shahnazi; Mohammad Asghari Jafarabadi; Vahid Khaze; Shakiba Pourasad Shahrak
Volume 13, Issue 3 , September 2016, , Pages 229-236
Abstract
Background: Preterm birth is a common problem in obstetrics. Objective: To measure maternal serum interlukin-6 in mothers with preterm uterine contractions and compare it with cervicovaginal interlukin-6 in the same women. Methods: In this cross-sectional study, we measured interlukin-6 in the sera and ...
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Background: Preterm birth is a common problem in obstetrics. Objective: To measure maternal serum interlukin-6 in mothers with preterm uterine contractions and compare it with cervicovaginal interlukin-6 in the same women. Methods: In this cross-sectional study, we measured interlukin-6 in the sera and cervicovaginal fluids of 86 women with preterm uterine contractions. All participants had an intact membrane. Interlukin-6 was measured by using ELISA method. Statistical analysis was done using U-Mann Whitney, Chi-Square and Kendall’s tests. Results: The mean and median (Quartile25, Quartile75) of interlukin-6 in cervicovaginal fluid was higher than maternal serum interlukin-6. There was a statically significant difference in the median of interlukin-6 in sera and cervicovaginal fluid (P<0.0001). There was no significant correlation between serum and cervicovaginal interlukin-6 (r=0.048, p=0.548). Conclusion: There was no significant correlation between serum and cervicovaginal interlukin-6 (r=0.048, p=0.548). Conclusion: We found no relationship between serum interlukin-6 and preterm labor and the maternal serum Interlukin-6 does not seem to be a suitable biomarker for predicting preterm delivery.
Zohreh Babaloo; Reza Khajir Yeganeh; Mehdi Farhoodi; Behzad Baradaran; Mohamadreza Bonyadi; Leila Aghebati
Volume 10, Issue 1 , March 2013, , Pages 47-54
Abstract
Background: Effector CD4+ T cell subsets play an important role in Multiple Sclerosis (MS). Interleukin-27 (IL-27) suppresses Th (Th1, Th2 and Th17) cells and dampens autoimmunity and tissue inflammation by promoting the generation of Type 1 regulatory T cells (Tr1). Objective: To identify the relative ...
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Background: Effector CD4+ T cell subsets play an important role in Multiple Sclerosis (MS). Interleukin-27 (IL-27) suppresses Th (Th1, Th2 and Th17) cells and dampens autoimmunity and tissue inflammation by promoting the generation of Type 1 regulatory T cells (Tr1). Objective: To identify the relative levels of IL-27 and IL-17A in MS disease. Method: In a case-control study, venous blood was collected from forty MS patients and forty-three healthy subjects as control group. Serum levels of IL-27 and IL-17A were measured by ELISA method. Results: A significant difference between serum IL-17A concentration in patients (120.68 ± 209.85 pg/ml) and control group (67.26 ± 117.76 pg/ml, p=0.016) was found. Serum IL-27 levels of the MS patients (159.7 ± 581.4 pg/ml) were significantly lower than control subjects (180.35 ± 507.84 pg/ml, p=0.001). Conclusion: Our findings show decreased levels of IL-27 against increasing IL-17A levels in patients group which may suggest the suppressive role of IL-27 on inflammatory process of MS.
Zohreh Babaloo; Farhad Babaie; Mehdi Farhoodi; Mohammadreza Aliparasti; Behzad Baradaran; Shohreh Almasi; Ahmad Hosseini
Volume 7, Issue 4 , December 2010, , Pages 1-1
Abstract
Bakground: Multiple sclerosis (MS) is a CD4+ T cell-mediated autoimmune disease affecting the central nervous system (CNS). It was previously believed that Th1 cells were pathogenic T cells in experimental autoimmune encephalomyelitis (EAE). However, the functional role of Th1 cells in EAE has been reconsidered ...
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Bakground: Multiple sclerosis (MS) is a CD4+ T cell-mediated autoimmune disease affecting the central nervous system (CNS). It was previously believed that Th1 cells were pathogenic T cells in experimental autoimmune encephalomyelitis (EAE). However, the functional role of Th1 cells in EAE has been reconsidered upon the discovery of IL-17- producing T cells which are consider as dominant effectors for inducing autoimmune tissue inflammation. Objective: The objective of this study was to assess the role of IL-17A and IL-17F in MS pathogenesis. Methods: We evaluated mRNA expression of IL-17A and IL-17F in thirty-five Iranian patients with relapsing–remitting MS (RRMS) and twenty-five healthy controls by Quantitative Real Time PCR. Results: The results of this study showed a twenty-fold increase in the expression of IL-17A mRNA in MS patients compared to the control group (p < 0.0001 ). IL-17F mRNA expression in MS patients was thirty three-times greater than control group (p = 0.0008). IL-17A mRNA expression in periphery was positively correlated with expression of IL-17F transcripts in MS patients and controls (p < 0.01 and p < 0.05, respectively). Conclusion: These results indicate the critical role of Th17- mediated cytokines in development of MS which classically has been considered as a Th1-mediated disorder. The results of this study showed, for the first time, the importance of IL-17F in MS immunopathogenesis.