Tahereh Mousavi; Alireza Salek Moghadam; Reza Falak
Volume 5, Issue 1 , March 2008, , Pages 57-63
Abstract
Background: There are many therapeutic methods for allergic conditions. CpG oli-gonucleotides play a critical role in immunity via the augmentation of Th1 and suppres-sion of Th2 responses. Objective: In the present study we aimed to estimate the effec-tiveness of intranasal administration of CpG ODN ...
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Background: There are many therapeutic methods for allergic conditions. CpG oli-gonucleotides play a critical role in immunity via the augmentation of Th1 and suppres-sion of Th2 responses. Objective: In the present study we aimed to estimate the effec-tiveness of intranasal administration of CpG ODN plus Chenopodium album allergen in allergic asthma compared with the administration of allergen alone and to find out how CpG ODN therapy is useful in the treatment of allergen induced asthma. Methods: BALB/c Mice were intraperitoneally and intranasally sensitized with allergenic extract precipitated on aluminum hydroxide. Therapy with CpG/Ag was performed intrana-sally. After antigenic challenge, a number of Immunologic variables such as serum IgE and IgG, systemic and local IL-10 and IFN-γ were studied in splenocytes, and lung tis-sue culture supernatants, respectively. Results: Our study indicated that intranasal ad-ministration of CpG/Ag had significant increases in both systemic and local levels of IL-10 and IFN-γ (p≤ 0.001), but showed no significant effect on the levels of IgE, IgG2a, and IgG1 in serum (p= 0.06). This study demonstrated that CpG ODN has thera-peutic effects not only on splenocytes but also on nasal lymphocytes to produce IFN-γ as a Th1 cytokine, and IL-10 as a regulatory cytokine. Conclusion: According to these data from the mouse model, we conclude that intranasal administration of CpG motifs before allergen exposure may be useful for the control of allergic asthma. Therefore, further investigations on humans using CpG motifs are recommended in order to modu-late the allergic effects of Chenopodium album as well as other regional allergens.
Tahereh Mousavi; Nahid Asadi; Majid Tebyanian
Volume 2, Issue 3 , September 2005, , Pages 166-171
Abstract
Background: The incidence of allergic and asthmatic diseases has been continuously increased in both industrial and developing countries. Extracts from various known allergens are used for the diagnostic and therapeutic purposes. Objective: To investigate the effects of an extract prepared from Chenopodium ...
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Background: The incidence of allergic and asthmatic diseases has been continuously increased in both industrial and developing countries. Extracts from various known allergens are used for the diagnostic and therapeutic purposes. Objective: To investigate the effects of an extract prepared from Chenopodium album (Ch.A.) pollen to induce allergic asthma in BALB/C mice. Methods: BALB/C mice were sensitized by i.p. injection of Ch.A. extract and alum, and an intratracheal instillation of the extract. The bronchoalveolar lavage (BAL) fluids were obtained by cannulating the trachea and lavaging the lungs and examined for eosinophilia. Splenocytes were incubated with Ch.A. extract and cell supernatants were examined for IL-4 and IL-5 by ELISA. Results: We demonstrated that Ch.A. extract treatment in mice increased serum levels of specific IgE and production of IL-4 and IL-5 from splenocytes. An airway eosinophilia was also demonstrated in mice. Conclusion: These results suggest that Ch.A. allergen extract is a potential agent in inducing characteristics of allergic asthma in a mouse model useful in investigational studies.
