Translational Insights on Lung Transplantation: Learning from Immunology
Mohamed Shehata
Ali Mohamed
Department of Thoracic Transplantation, University Clinic Essen, Essen, Germany
author
text
article
2015
eng
The introduction of ex vivo lung perfusion (EVLP) in the practice of lung transplantation has allowed the reconditioning of the marginal grafts and their conversion into transplantable grafts. In addition, EVLP can provide a platform for the application of various preventive measures to decrease the incidence of post-transplant complications. While the Toronto team targets the attenuation of the cytokine production within the graft through gene therapy to up-regulate IL-10, other measures could be applied to achieve significant attenuation of the cytokine load of the graft. This manuscript provides a short overview on the importance of the attenuation of the cytokine production within the transplanted lung grafts and some possible strategies to achieve this goal.
Iranian Journal of Immunology
Shiraz Institute for Cancer Research
1735-1383
12
v.
3
no.
2015
156
165
https://iji.sums.ac.ir/article_16745_445230c748ca8818ae1ec56063604990.pdf
Patients with Active Rheumatoid Arthritis Have Lower Frequency of nTregs in Peripheral Blood
Hamid-Reza
Zare
Department of Biology
author
Mojtaba
Habibagahi
Fars Science and Research Branch, Islamic Azad University
author
Akbar
Vahdati
Department of Biology
author
Zahra
Habibagahi
Department of
Internal Medicine, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
author
text
article
2015
eng
Background: Patients with rheumatoid arthritis (RA) suffer from wide ranges of autoimmune reactions in joints. The mechanism of which is generally unknown and maybe associated with Treg deregulation. Objective: To compare the frequency of nTregs in peripheral blood of patients with active rheumatoid disease with healthy individuals. Methods: Twenty five newly diagnosed patients with active RA disease were selected based on the clinical and laboratory criteria before starting their therapies. Treg cells in peripheral blood samples were enumerated by immune staining and flowcytometry analysis. Results: Clinical and laboratory results were in favor of active disease in all the studied patients although they showed variations in Disease Activity Score-28 (DAS-28). Compared to the healthy controls, RA patients had significantly lower frequency of CD4+ CD25hi or CD4+ CD25+ FoxP3+ natural regulatory T cells. In spite of that, there were no significant differences between patients and healthy controls in respect to the CD4/CD8 ratio. Interestingly, more CD4+ CD25- FoxP3+ cells were found in peripheral blood of patients. The frequencies of the Tregs did not show strong associations with the DAS-28. Conclusion: We showed lower abundance of nTregs in peripheral blood of RA patients which highlights the significance of these cells in RA.
Iranian Journal of Immunology
Shiraz Institute for Cancer Research
1735-1383
12
v.
3
no.
2015
166
175
https://iji.sums.ac.ir/article_16746_49d6957f732b948e2c3a6a1829a80278.pdf
Multiple Low Doses of 5-Fluorouracil Diminishes Immunosuppression by Myeloid Derived Suppressor Cells in Murine Melanoma Model
Afshin
Namdar
Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan
author
Hamid Reza
Mirzaei
Department of Immunology, School of Medicine
author
Farhad
Jadidi-Niaragh
Department of Pathobiology, School of Public Health
author
Mahboubeh
Ashourpour
Department of Pathobiology, School of Public Health
author
Maryam
Ajami
Department of Immunology, School of Medicine, Tarbiat Modares University,
Tehran, Iran
author
Jamshid
Hadjati
Department of Immunology, School of Medicine
author
Abbas
Rezaei
Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan
author
text
article
2015
eng
Background: Melanoma progression and metastasis is suggested to be mediated by increased accumulation of myeloid derived suppressor cells. Various chemotherapeutic drugs such as 5-Fluorouracil in single low concentration have the capacity, at least in part, to reverse tumor progression by reducing myeloid derived suppressor cellsmediated immunosuppression. Objective: To assess whether multiple low doses of 5- fluorouracil could repress myeloid derived suppressor cells in low frequency and, in turn, could enhance anti-tumor responses and promote a more prolonged survival in a murine melanoma model. Methods: Fifty milligram per kilogram body weight dose of 5-Flourouracil was administered intraperitoneally 4 times with 3-day intervals to C57BL/6 mice after B16 melanoma tumor models were established. The frequency and suppressive functions of myeloid derived suppressor cells and induction of anti-tumor CD8+ T cells as well as tumor growth and survival were evaluated in drug treated and untreated mice. Results: Our results demonstrated that this therapeutic strategy increases the overall mice survival (p≤0.01) and induces melanoma-specific CD8+T cell immunity (p≤0.01) by reducing the frequency of myeloid derived suppressor cells (p≤0.01) as well as their immune suppressive functions (p≤0.05). Conclusion: Altogether, our data suggest that 5-fluorouracil in multiple low regimens might be used to overcome tumor immunosuppression and improve the efficacy and outcome of antitumor immune responses in a mouse model.
Iranian Journal of Immunology
Shiraz Institute for Cancer Research
1735-1383
12
v.
3
no.
2015
176
187
https://iji.sums.ac.ir/article_16747_80916eb8cab5a3f80311da2f3704a981.pdf
Subunit Vaccine Preparation of Bovine Rotavirus and Its Efficacy in Mice
Wei
Suocheng
The key Bio-Engineering and Technology Laboratory of Nationality Commission
author
Che
Tuanjie
Lanzhou Baiyuan Company for Gene
Technology, Lanzhou, China
author
Song
Changjun
Life Science and
Engineering College, Northwest University for Nationalities
author
Tian
Fengling
Life Science and
Engineering College, Northwest University for Nationalities
author
Ma
Zhongren
The key Bio-Engineering and Technology Laboratory of Nationality Commission
author
text
article
2015
eng
Background: Rotaviruses (RV) are important viral diarrheal agents in calves. Vaccination is an optimum measure to prevent bovine rotaviruses (BRV) infection. However, little research on BRV VP7 vaccine has been done and currently there is no BRV vaccine. Objective: To prepare a subunit vaccine of BRV and investigate its efficacy. Methods: Total RNA was extracted from MA104 cells infected with bovine rotavirus (BRV) strain GSB01. BRV VP7 gene was amplified using real time fluorescence quantitative PCR (qPCR). The pEASY-T3-VP7 plasmid was digested using Hind Ⅲ and BamHI restriction endonucleases, then recombined into the prokaryotic expression vector pET32a. The pET32a-VP7 and pET32a-VP7-LTB (heatlabile enterotoxin B subunit) were transformed into BL21 (DE3) competent cells of Escherichia coli, respectively, and induced with IPTG, then analyzed using SDS-PAGE. Sixty mice were randomly divided into three groups (n=20). Group A mice was used as His-tag control and mice in group B and C were inoculated with pET32a-VP7 and pET32a-VP7-LTB, respectively. VP7 IgG antibody titers and protection efficiency of pET32a-VP7-LTB were further determined in neonatal mice challenged with GSB01 BRV strain. Results: SDS-PAGE analysis showed that the pET32a-VP7 was highly expressed in the BL21 (DE3) cells. PET32a-VP7 and pET32a-VP7-LTB protein could promote VP7 IgG antibody titer(8.33×103 vs. 17.26×103)in mice. Immunization protection ratios of pET32a-VP7 and pET32a-VP7-LTB proteins in the neonatal mice were 86.4% and 91.7%, respectively. Conclusion: The fusion protein of pET32a-VP7- LTB had excellent immunogenicity and protected mice from BRV infection. Our findings can be used for further developing of a high-efficiency subunit vaccine of BRV.
Iranian Journal of Immunology
Shiraz Institute for Cancer Research
1735-1383
12
v.
3
no.
2015
188
197
https://iji.sums.ac.ir/article_16748_0b28fdaa4612989c6943d9f7296c62e0.pdf
Antigenic Variation of the Haemagglutinin Gene of the Influenza A (H1N1) pdm09 Virus Circulating in Shiraz, February-April 2013
Forough
Tavakoli
Department of Bacteriology and Virology, Shiraz University of Medical Sciences, Shiraz
author
Afagh
Moattari
Department of Bacteriology and Virology, Shiraz University of Medical Sciences, Shiraz
author
Mahmoud
Shamsi Shahr Abadi
Department of
Virology, Tehran University of Medical Sciences, Tehran
author
Mohammad Rahim
Kadivar
Department of Pediatrics, Nemazee Hospital,
Shiraz University of Medical Sciences, Shiraz, Iran
author
Nastaran
Khodadad
Department of Bacteriology and Virology, Shiraz University of Medical Sciences, Shiraz
author
Neda
Pirbonyeh
Department of Bacteriology and Virology, Shiraz University of Medical Sciences, Shiraz
author
Amir
Emami
Department of Bacteriology and Virology, Shiraz University of Medical Sciences, Shiraz
author
text
article
2015
eng
Background: A new pandemic influenza A (H1N1) emerged in April 2009, causing considerable morbidity and mortality. Since mutations in the haemagglutinin (HA) may influence the antigenicity and pathogenicity of the virus, continued epidemiological and molecular characterization for the effective control of pandemic flu and developing of more appropriate vaccine is crucial. Objective: To monitor the molecular evolution of A (H1N1) pdm09 viruses in a specific time period in Shiraz, Southern Iran. Methods: A total of 200 samples were collected from February-April 2013. HA gene of the isolates was amplified and sequenced. Phylogenetic analysis of the HA gene was performed. Results: Out of 200 samples, a total of 77 (38.5%) samples were confirmed as A (H1N1) pdm09 virus using Real-time PCR method. Nucleotide similarity of our study strains with respect to reference strain A/California/07/2009 (H1N1) was 97.5%-98.5%. Phylogenetic analysis of our study strains indicated that the dominant A (H1N1) pdm09 clade was clade 7 and the dominant genetic group in circulating strains in Shiraz was genetic group 6. Some of our study strains showed substitutions at or in the vicinity of the antigenic sites of the HA1 region which may affect the efficacy of the vaccine. Conclusion: Our study strains showed a high homology to the vaccine strain. Our findings confirm the genetic variability of influenza A (H1N1) pdm09 and highlight the necessity of continuous molecular study of the virus for effective management of influenza.
Iranian Journal of Immunology
Shiraz Institute for Cancer Research
1735-1383
12
v.
3
no.
2015
198
208
https://iji.sums.ac.ir/article_16749_216d1afdb2adf1dcf00d08c1aaf650ec.pdf
Report on the First Survey of Iranian Patients with Hereditary Angioedema
Shervin
Shahinpour
Research Center for Immunod eficiencies, Pediatrics Ce nter of Excel lence, Children's Medical C enter,
Tehran University of Medical Sciences, Tehran
author
Marzieh
Tavakol
Department of Allergy and Clinical Immunology, Shahid
Bahonar Hospital, Alborz U niversity of Medical Sciences, Karaj, Iran
author
Hassan
Abolhass ani
Research Center for Immunod eficiencies, Pediatrics Ce nter of Excel lence, Children's Medical C enter,
Tehran University of Medical Sciences, Tehran
author
Payam
Mohammadinejad
Research Center for Immunod eficiencies, Pediatrics Ce nter of Excel lence, Children's Medical C enter,
Tehran University of Medical Sciences, Tehran
author
Masoud
Movahedi
Research Center for Immunod eficiencies, Pediatrics Ce nter of Excel lence, Children's Medical C enter,
Tehran University of Medical Sciences, Tehran
author
Saba
Arshi
Allergy and Clinical Immunology Department of RasolE-Akram
Hospital, Iran University of Medical Sciences, Tehran, Iran
author
Asghar
Aghamohammadi
Research Center for Immunod eficiencies, Pediatrics Ce nter of Excel lence, Children's Medical C enter,
Tehran University of Medical Sciences, Tehran
author
text
article
2015
eng
Background: Hereditary angioedema (HAE) is a rare autosomal dominant primary immunodeficiency with complement system defect characterized by recurrent episodes of angioedema involving the skin or mucosa of the upper respiratory and gastrointestinal tracts. Objective: To characterize the clinical and laboratory data of hereditary angioedema in Iran. Methods: Patients with probable diagnosis of angioedema were enrolled in this study. Demographic and clinical data were documented in the designed questionnaire including history of attacks, triggering factors and laboratory data such as C4, C1 esterase inhibitor level and function. Results: Among 63 patients who were clinically suspicious for angioedema (23 males and 40 females), 8 cases (12.7%) were diagnosed with HAE. Among these 8 HAE patients, 3 were diagnosed with HAE type 1 and five patients were diagnosed with HAE type 2. The mean ages of HAE type 1 and type 2 patients were 25.6 ± 13.5 and 22.4 ± 12.32 years. The mean age of onset in HAE type 1 group was 8 ± 5 years and in HAE type 2 group was 18.8 ± 11.84 years. The mean diagnosis delay was 17.6 years in HAE type 1 patients and 2.6 years in HAE type 2. The most common clinical manifestation was facial swelling presented in all HAE patients followed by swelling of extremities which was present in 7 patients with HAE. Conclusion: The clinical criteria of the Iranian patients with HAE were consistent with the known clinical patterns of the disease.
Iranian Journal of Immunology
Shiraz Institute for Cancer Research
1735-1383
12
v.
3
no.
2015
209
218
https://iji.sums.ac.ir/article_16750_b43d2e2b50ffc5f7e055a129e4244121.pdf
A Case of Probable MHC Class II Deficiency with Disseminated BCGitis
Soheyla
Alyasin
Clinical Immunology and Allergy, Allergy Research Center
author
Farhad
Abolnezhadian
Department of Pediatrics, Division of
Immunology and Allergy, Namazi Hospital, Shiraz University of Medical Sciences, Shiraz
author
Maryam
Khoshkhui
Department of
Clinical immunology and Allergy, Mashhad University of Medical Science , Mashhad, Iran
author
text
article
2015
eng
Major histocompatibility complex (MHC) class II deficiency is a primary immunodeficiency disease characterized by abnormality of MHC class II molecules surface expression on peripheral blood lymphocytes and monocytes. Clinical manifestations include extreme susceptibility to viral, bacterial, and fungal infections but the immunodeficiency is not as severe as SCID (severe combined immunodeficiency), as evidenced by failure to develop disseminated infection after BCG vaccination. Therefore, MHC II deficiency with BCGosis, that is disseminated BCGitis, is not reported commonly. We report an interesting case of BCGosis after vaccination that was diagnosed to have probable MHC II deficiency.
Iranian Journal of Immunology
Shiraz Institute for Cancer Research
1735-1383
12
v.
3
no.
2015
219
225
https://iji.sums.ac.ir/article_16751_c58b93ba912167126ee738d7e1d562eb.pdf