@article { author = {Abdullahi, Masooma and Ranjbaran, Reza and Alyasin, Soheaila and Keshavarz, Zeinab and Ramezani, Amin and Behzad-Behbahani, Abbas and Sharifzadeh, Sedigheh}, title = {Expression of Basophil Activation Markers in Pediatric Asthma}, journal = {Iranian Journal of Immunology}, volume = {13}, number = {1}, pages = {27-36}, year = {2016}, publisher = {Shiraz Institute for Cancer Research}, issn = {1735-1383}, eissn = {1735-367X}, doi = {}, abstract = {Background: Asthma is very common in children and its diagnosis is based on clinical manifestations, which can be misdiagnosed as other respiratory diseases with similar signs and symptoms. Objective: To analyze the expression of ST2L and CD203c in the diagnosis of pediatric asthma. Methods: Basophils were purified from whole blood samples of patients and healthy controls using Ficol-Paque gradient and Basophil Isolation Kit. RNA extraction was done by RNX-Plus solution and after synthesis of cDNA, the gene expression was analyzed by means of real time PCR. Results: Patients expressed significantly higher levels of CD203c than healthy controls (p=0.01). Although there was an increase in the transcription level of ST2L gene in patients, the results were not statistically significant compared to those obtained from the healthy controls (p>0.05). A Specificity of 60% and a sensitivity of 73% were foundusing ROC curve for CD203c expression. Patients with positive family history of asthma exhibited more CD203c and ST2L expression (p<0.05). Conclusion: It is proposed that determining CD203c expression by real time PCR may be an effective technique for diagnosis of pediatric asthma.}, keywords = {Asthma,Basophil,CD203c,ST2L}, url = {https://iji.sums.ac.ir/article_16725.html}, eprint = {https://iji.sums.ac.ir/article_16725_ef993c0c8c3a2884235ba1b100285557.pdf} }