@article { author = {Ahmadi, Kazem and Riazipour, Majid}, title = {Ganoderma Lucidum Induces the Expression of CD40/CD86 on Peripheral Blood Monocytes}, journal = {Iranian Journal of Immunology}, volume = {6}, number = {2}, pages = {87-91}, year = {2009}, publisher = {Shiraz Institute for Cancer Research}, issn = {1735-1383}, eissn = {1735-367X}, doi = {}, abstract = {Background: The major immuno-modulating effects of Ganoderma lucidum include mitogenicity and activation of immune effector cells such as T cells, macrophages and natural killer cells resulting in the production of cytokines. Objective: The purpose of this study was to evaluate the expression of CD40 and CD80 by G. lucidum-treated human peripheral blood mononuclear cells. Methods: Monocytes were isolated and incubated at 37°C and 5% CO2 for 24 h and 48 h in the presence or absence of different concentrations of G. lucidum. Cells were then incubated with labelled monoclonal antibodies against CD14, CD40 and B7-1(CD80) molecules utilizing standard protocols, and analyzed by flow cytometry. Results: The results showed that incubation of monocytes with G. lucidum led to marked enhancement of CD40 and B7-1 expression in a dose- and time- dependent manner (p<0.001). G. lucidum was more effective in enhancing the expression of CD80 and CD40 molecules of cells obtained from females than male donors (p<0.001). Conclusion: G. lucidum enhanced the expression of CD40 and CD80 molecules on peripheral blood monocytic cells derived from both sexes in a dose-dependent manner, with a preferential higher effect on cells obtained from female donors.}, keywords = {Ganoderma lucidum,Monocytes,CD14,CD40,CD80}, url = {https://iji.sums.ac.ir/article_17078.html}, eprint = {https://iji.sums.ac.ir/article_17078_106d0b33fd2e6aab98016b08dd503c9f.pdf} }