@article { author = {Cataño Cañizalez, Yolanda and Uresti Rivera, Edith and Garcia Jacobo, Rocio and Portales Perez, Diana and Bastian, Yadira and Rodriguez Rivera, J and Gonzalez Amaro, Roberto and Enciso Moreno, Jose and Garcia Hernandez, Mariana}, title = {Increased Levels of AIM2 and Circulating Mitochondrial DNA in Type 2 Diabetes}, journal = {Iranian Journal of Immunology}, volume = {15}, number = {2}, pages = {142-155}, year = {2018}, publisher = {Shiraz Institute for Cancer Research}, issn = {1735-1383}, eissn = {1735-367X}, doi = {10.22034/iji.2018.39378}, abstract = {Background: Chronic inflammation has critical role in Type 2 diabetes (T2D), in which IL-1β contributes in insulin resistance and beta cell dysfunction. The activation of NLRP3 and AIM2 by endogens ligands, such as mtDNA can lead to the release of active form of IL-1β. Objective: To evaluate AIM2 expression and activation as well as circulating mtDNA levels in T2D patients. Methods: AIM2 expression was analyzed by flow cytometry, it’s activity was assessed by measuring in vitro release of IL-1β induced by Poly (dA:dT), and mtDNA copy number was determined by quantitative real-time polymerase chain reaction. Results: Increased percent of AIM2+ cells were detected in monocytes from patients with T2D. Moreover, increased levels of IL-1β in monocytes cultures from T2D patients compared to healthy controls were observed. Also, association between AIM2+ cells and hyperglycemia (r=0.4385, P=0.0095) and triglycerides levels (r=0.5112, P=0.002) and waist-hip ratio (r=0.4710, P=0.0049) were detected. Likewise, the mtDNA copy number was augmented in T2D patients compared to control group. The mtDNA copies number was associated with body mass index (r=0.4231, P=0.0008) and TNF-α levels (r=0.5231, P=0.0005). In addition, increased levels of IL-12p70, TNF-a, IL-10, IL-6, IL-8 and IL-1β were detected in a serum from T2D patients. Conclusion: These results suggest the involvement of AIM2 and mtDNA in the inflammatory process seen in T2D.}, keywords = {AIM2,Cell-free mtDNA,IL-1β,Inflammasome,Type 2 Diabetes}, url = {https://iji.sums.ac.ir/article_39378.html}, eprint = {https://iji.sums.ac.ir/article_39378_a3926cd07e0a2ff5c0ab76d00ee35d89.pdf} }