%0 Journal Article %T Detection of IL-20R1 and IL-20R2 mRNA in C57BL/6 Mice Astroglial Cells and Brain Cortex Following LPS Stimulation %J Iranian Journal of Immunology %I Shiraz Institute for Cancer Research %Z 1735-1383 %A Abd Nikfarjam, Bahareh %A Ebtekar, Massoumeh %A Sabouni, Farzaneh %A Pourpak, Zahra %A Kheirandish, Maryam %D 2013 %\ 06/01/2013 %V 10 %N 2 %P 62-69 %! Detection of IL-20R1 and IL-20R2 mRNA in C57BL/6 Mice Astroglial Cells and Brain Cortex Following LPS Stimulation %K Astroglial Cells %K IL-20R1 %K IL-20R2 %K LPS %K mice %R %X Background: Astrocytes, which comprise ~90% of overall brain mass, are involved in brain immunity. These cells represent the non-professional class of CNS-resident APCs and may promote or inhibit CNS inflammation depending on the cytokines they secrete. IL-10 family of cytokines and their receptors, IL-20R1 and IL-20R2, may have a role in shifting astrocytes to a neuroprotective or neurodegenerative function. Objective: To address the expression of IL-20R1 and IL-20R2 cytokine receptors in astrocytes and brain cortex of C57BL/6 mice. Methods: We investigated the expression of IL-20R1 and IL-20R2 in C57BL/6 mice astroglial cells and brain cortex in response to lipopolysaccharide (LPS), using reverse-transcription polymerase chain reaction (RTPCR) method. Results: Astrocytes were able to express IL-20R1 and IL-20R2 mRNA not only in response to LPS stimulation but also in the absence of LPS. Furthermore, we found the expression of IL-20R1 and IL-20R2 mRNA in the cortex of adult C57BL/6 mice. Conclusions: IL-20R1 and IL-20R2 are constitutively expressed in the brain. Since most neuropathological processes involve astrocytes and inflammatory cytokines, these findings have important implications for future therapeutic strategies. %U https://iji.sums.ac.ir/article_16809_d4ca233c60c0b6c779d7564d3bdad5fb.pdf