%0 Journal Article %T IL-10 and IL-12 Production in Response to Mycobacterium Tuberculosis Total Lipid Antigens in Multidrug-Resistant Tuberculosis %J Iranian Journal of Immunology %I Shiraz Institute for Cancer Research %Z 1735-1383 %A Shahemabadi, Ali Shams %A Zavaran Hosseini, Ahmad %A Shaghasempour, Shapour %A Masjedi, Mohammad Reza %A Rayani, Majid %A Shams, Majid %A Esphandyari, Nasrin %A Pouramiri, Majid %D 2010 %\ 03/01/2010 %V 7 %N 1 %P 57-63 %! IL-10 and IL-12 Production in Response to Mycobacterium Tuberculosis Total Lipid Antigens in Multidrug-Resistant Tuberculosis %K IL-10 %K IL-12 %K Lipid %K Macrophage %K Monocyte %K Multidrug Resistant Tuber-culosis %R %X Background: Mycobacterium tuberculosis lipid antigens take part in pathogenicity of the bacterium but the response of monocytes/macrophages to these antigens in tubercu-losis is not well known. Objective: The aim of current investigation was to study the M. tuberculosis lipid antigens in tuberculosis pathogenesis. Methods: In the present study M. tuberculosis lipid antigens were extracted. Monocytes and macrophages from mul-tidrug-resistant tuberculosis (MDR-TB), TB patients, asymptomatic healthy individuals with positive tuberculin skin test positive and healthy individuals with negative tubercu-lin skin test were collected using magnetic cell sorting. The cells were stimulated by M. tuberculosis total lipid antigens and IL-12 and IL-10 in their supernatants were meas-ured by enzyme-linked immunosorbent assay. Results: The IL-12 production by mono-cytes in response to M. tuberculosis total sonicate antigens in the MDR-TB patients did not show a considerable difference with the PPD positive healthy subjects, whereas in the active TB patients, IL-12 levels significantly decreased (p<0.05). IL-10 production by monocytes in TB patients in response to total lipid antigens showed a significant in-crease in comparison to MDR-TB patients and healthy individuals. Conclusion: In the MDR-TB patients, IL-10 and IL-12 production by monocytes in response to M. tubercu-losis lipid antigens are similar to the healthy subjects. %U https://iji.sums.ac.ir/article_17040_b0490ee8da84ed4e17d44b52c0ca94af.pdf