TY - JOUR ID - 16834 TI - Interleukin-17 Gene Expression and Serum Levels in Children with Severe Asthma JO - Iranian Journal of Immunology JA - IJI LA - en SN - 1735-1383 AU - Alyasin, Soheila AU - Karimi, Mohammad Hossein AU - Amin, Reza AU - Babaei, Maryam AU - Darougar, Sepideh AD - Allergy Research Center AD - Transplant Research Center Y1 - 2013 PY - 2013 VL - 10 IS - 3 SP - 177 EP - 185 DO - N2 - Background: IL-17 is a major cytokine player in T cell mediated leukocyte associated inflammation. IL-17 is also recognized to participate in the pathophysiology of asthma. Objective: To determine the role of IL-17 in predicting severe asthma. Methods: We obtained serum samples from asthmatic children under the age of 5-year in three different groups of mild (n=33), moderate (n=28) and severe (n=32) persistent asthma. IL-17 serum concentrations and mRNA expression were determined by ELISA and real time PCR assays, respectively. Results: Serum IL-17 concentrations were significantly higher in patients with severe asthma than the other two groups of children with mild and moderate disease (p=0.00). Mean serum IL-17 values were 142.04 pg/ml in mild group, 180.4 pg/ml in moderate group and 251.25 pg/ml in severe group. IL-17 mRNA levels were also significantly elevated in severe asthmatic patients compared to mild and moderate asthmatic children (p=0.00). Conclusion: Our data reveal an increase in the serum IL-17 concentrations and IL-17 mRNA expressions in children with severe asthma compared to those with mild and moderate forms of the disease. UR - https://iji.sums.ac.ir/article_16834.html L1 - https://iji.sums.ac.ir/article_16834_dff4322a2b35feb8b1cd4a2969316d38.pdf ER -