TY - JOUR ID - 16858 TI - Differential WNT Gene Expression in Various Subtypes of Acute Lymphoblastic Leukemia JO - Iranian Journal of Immunology JA - IJI LA - en SN - 1735-1383 AU - Memarian, Ali AU - Vosough, Parvaneh AU - Asgarian-Omran, Hossein AU - Tabrizi, Mina AU - Shabani, Mahdi AU - Shokri, Fazel AD - Department of Immunology, School of Public Health AD - Clinic of Hematology and Oncology, Ali-Asghar Hospital, Faculty of Medicine AD - Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences AD - Monoclonal Antibody Research Center, Avicenna Research Institute, Tehran, Iran AD - Department of Immunology, School of Public Health Y1 - 2012 PY - 2012 VL - 9 IS - 1 SP - 61 EP - 71 KW - Acute Lymphoblastic Leukemia KW - Immunophenotype KW - RT-PCR KW - Wnt DO - N2 - Background: Dysregulation of WNT signaling has been reported in many malignancies. Objective: This study was conducted to investigate the expression pattern of 14 members of the WNT gene family in different immunophenotypic subtypes of ALL. Methods: Semi-quantitative RT-PCR was performed on samples from 71 ALL patients and 36 age-matched healthy individuals. The ALL patients were categorized into BALL (76%), T-ALL (22.6%) and mixed lineage (1.4%) and the B-ALL cases were further classified into pro-B, pre-BI, pre-BII and immature/mature-B based on immunophenotypic results. Results: Among the WNT genes, WNT-7B (p=0.026), WNT-9A (p=0.020) and WNT-16B (p=0.023) were significantly over-expressed, whereas WNT- 2B (p=0.033), WNT-5A (p=0.016), WNT-7A (p<0.0001) and WNT-10A (p<0.0001) were down-regulated in B-ALL. Among the T-ALL subtype, however, significant down-regulation of WNT-2B, WNT-5B, WNT-7A, WNT-10A and WNT-11 was evident. Comparison between B-ALL subtypes showed significant over-expression of WNT-7B, WNT-9A and WNT-5B in certain subtypes. Conclusion: Our results suggest contribution of the WNT genes in leukemogenesis of ALL. UR - https://iji.sums.ac.ir/article_16858.html L1 - https://iji.sums.ac.ir/article_16858_39dde9034dbbdff779b5d4c11d967948.pdf ER -