TY - JOUR ID - 17097 TI - Immunophenotypic Characterization of the Leukemic B-cells from Iranian Patients with Chronic Lymphocytic Leukemia: Association between CD38 Expression and Disease Progression JO - Iranian Journal of Immunology JA - IJI LA - en SN - 1735-1383 AU - Farasangi, Mohammad Hojjat AU - Jeddi-Tehrani, Mahmood AU - Razavi, Seyed Mohsen AU - Sharifian, Ramazan Ali AU - Shamsian Khoramabadi, Ahmad AU - Rabbani, Hojatollah AU - Shokri, Fazel AD - Department of Immunology, School of Public Health, Medical Sciences /Tehran University, Tehran, Iran AD - Immune and Gene Therapy Lab, Cancer Center Karolinska, Karolinska Hospital, Stockholm, Sweden AD - Clinic of Hematology and Oncology, Firozgar Hospital, Faculty of Medicine, Iran University of Medical Sciences AD - Clinic of Hematology and Oncology, Vali-Asr Hospital, Faculty of Medicine, Medical Sciences/ University of Tehran AD - Department of Immunology, School of Public Health, Medical Sciences /Tehran University, Tehran, Iran Y1 - 2008 PY - 2008 VL - 5 IS - 1 SP - 25 EP - 35 KW - Immunophenotyping KW - B-CLL KW - Indolent KW - Progressive KW - CD38 DO - N2 - Background: Patients with B-cell chronic lymphocytic leukemia (B-CLL) have hetero-geneous clinical courses, thus several biological parameters need to be added to the cur-rent clinical staging systems to predict disease outcome. Recent immunophenotypic stud-ies performed mainly in Western populations have demonstrated the prognostic value of CD38 and ZAP-70 expression in B-CLL. Objectives: To investigate the expression pat-tern of a variety of membrane antigens on leukemic cells from Iranian patients with CLL and to find out if there are any differences in the expression of these markers between in-dolent and progressive groups. Methods: In the present study, peripheral blood samples from 87 Iranian patients with B-CLL were analysed by flow cytometry. Results: In all cases, the neoplastic cells displayed B-CLL phenotype (CD5+/CD19+/sIg+). The vast ma-jority of the cases expressed CD23, but failed to stain for CD3 or CD14. The leukemic cells of most patients expressed CD27 (84/87, 95.4%) and CD45RO (74/87, 83.9%) molecules, suggesting a memory B-cell phenotype. Comparison between the indolent (n=42) and progressive (n=37) patients revealed significantly higher frequency and inten-sity of CD38 expression in progressive group (40.5%) compared to indolent (11.9%) pa-tients (p<0.05). None of the other membrane antigens were differentially expressed in these two groups of patients. Conclusion: Our results obtained in an Asian ethnic popula-tion confirm and extend previous findings obtained from Western populations regarding the association of CD38 expression and disease progression in B-CLL. UR - https://iji.sums.ac.ir/article_17097.html L1 - https://iji.sums.ac.ir/article_17097_bb2db15f8f0c3df2a4e88aedd2a976c8.pdf ER -