TY - JOUR ID - 39309 TI - Rapamycin Inhibits Expansion of Cord Blood Derived NK and T Cell JO - Iranian Journal of Immunology JA - IJI LA - en SN - 1735-1383 AU - Zare, Monireh AU - Valipour, Behnaz AU - Mohammadi, Seyede Momeneh AU - Nouri, Mohammad AU - Movassaghpour, Aliakbar AU - Nozad Charoudeh, Hojjatollah AD - Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran AD - Department of Biochemistry, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran AD - Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran Y1 - 2017 PY - 2017 VL - 14 IS - 3 SP - 192 EP - 199 KW - Cytokine KW - mTOR KW - NK and T Cells KW - Rapamycin DO - N2 - Background: The mammalian target of rapamycin (mTOR) is important in hematopoiesis. Despite the central role of mTOR in regulating the differentiation of immune cells, the effect of mTOR function on cord blood mononuclear cells is yet to be defined. Objectives: To evaluate the effect of mTOR inhibition, using rapamycin on the proliferation and apoptosis of cord blood mononuclear cells, as well as on the B and T cell expansion. Methods: Cord blood mononuclear cells were cultured in the presence of IL-2, IL-7 and IL-15 cytokines and inhibited by rapamycin for 14 days. The harvested cells were evaluated at distinct time points by flow cytometry. Results: The mTOR expression decreased in the presence of rapamycin on day 14. Inhibition of mTOR reduced the proliferation of the cord blood mononuclear cells, yet did not influence apoptosis. Moreover, the number of T and NK cells was significantly reduced in the presence of rapamycin, while no change was observed in the B cell expansion. Conclusion: mTOR signaling plays a crucial part in cord blood derived NK and T cells expansion. UR - https://iji.sums.ac.ir/article_39309.html L1 - https://iji.sums.ac.ir/article_39309_c585f948c43dc9f673375f0181d46046.pdf ER -