TY - JOUR ID - 45566 TI - Hepcidin Induces M1 Macrophage Polarization in Monocytes or THP-1 Derived Macrophages JO - Iranian Journal of Immunology JA - IJI LA - en SN - 1735-1383 AU - Liu, Enna AU - Li, Zheng AU - Zhang, Yan AU - Chen, Kuisheng AD - Department of Tumor Pathology, Luohe Medical College, Henan, China AD - Yi-Chuang Institute of Biotechnology Industry, Beijing, China AD - College of Basic Medicine, Zhengzhou University, Henan, China Y1 - 2019 PY - 2019 VL - 16 IS - 3 SP - 190 EP - 199 KW - Hepcidin KW - Macrophage KW - Polarization DO - 10.22034/iji.2019.80270 N2 - Background: Macrophage polarization plays a critical role in determining the inflammatory states. Hepcidin is a key negative regulator of iron homeostasis and functions. Although hepcidin has been shown to affect ferroportin expression in macrophages, whether it affects macrophage polarization is still largely unknown. Objective: To address whether hepcidin induces macrophage polarization. Methods: The expression of iNOS and CD206, and the ratio of IFN-γ vs IL-4 in THP-1 derived macrophages upon hepcidin stimulation were evaluated. Further detected was the percentage of CD16+ M1, CD23+ M1, CD10+ M2 and CCL22+ M2 cells in monocyte derived macrophages. Results: M1 associated molecules were increased in hepcidin-treated cells, yet M2 associated molecules were increased when hepcidin was neutralized. Concomitantly, we observed a significant increase in IRF3 phosphorylation in hepcidin-stimulated cells. However, STAT6 phosphorylation with hepcidin was neutralized. Conclusion: Hepcidin is able to induce macrophage polarization towards M1 type, and might be utilized as a potential M1 macrophage agonist in clinical practice. UR - https://iji.sums.ac.ir/article_45566.html L1 - https://iji.sums.ac.ir/article_45566_e4dea11643d0b6a6fb4bbe2223235464.pdf ER -