Shiraz Institute for Cancer Research
Iranian Journal of Immunology
1735-1383
15
3
2018
09
01
Role of Interleukin-37 in Inflammatory and Autoimmune Diseases
165
174
39386
10.22034/iji.2018.39386
EN
Xiaoying
Wang
Department of Immunology, Medical School of Ningbo University, Ningbo, China
Keye
Xu
Department of Immunology, Medical School of Ningbo University, Ningbo, China
Sisi
Chen
Department of Immunology, Medical School of Ningbo University, Ningbo, China
Yan
Li
Department of Immunology, Medical School of Ningbo University, Ningbo, China
0000-0001-5158-6636
Mingcai
Li
Department of Immunology, Medical School of Ningbo University, Ningbo, China
0000-0003-3910-6964
Journal Article
2018
09
24
Interleukin-1 family 7 (IL-1F7) is a novel member of IL-1F cytokines. IL-1F7 is more commonly known as IL-37. IL-37 join the α-subunit of the IL-18 receptor, or IL-18 binding protein (IL-18BP), and binding of these proteins can enhance the IL-18 suppression. IL-37 also translocates to the cell nucleus and affects gene transcription. IL-37 inhibits the phosphorylation of p38 mitogen-activated protein kinases. Almost all reports showed that IL-37 has remarkable anti-inflammatory activity. IL-37 plays an important role in a variety of inflammatory and autoimmune diseases, as well. Recently, studies demonstrated that the expression of IL-37 is abnormal in many diseases such as inflammatory bowel diseases, inflammatory respiratory diseases, atherosclerosis, hepatitis, obesity, contact hypersensitivity, Graves’ disease, rheumatoid arthritis, ankylosing spondylitis, systemic lupus erythematosus, multiple sclerosis, psoriasis, and Behcet's disease. Here, we will review the biological characteristics of IL-37 and its key roles in various inflammatory and autoimmune diseases.
Shiraz Institute for Cancer Research
Iranian Journal of Immunology
1735-1383
15
3
2018
09
01
T Helper Cells Profile and CD4+CD25+Foxp3+Regulatory T Cells in Polycystic Ovary Syndrome
175
185
39387
10.22034/iji.2018.39387
EN
Fatemeh
Nasri
Department of Immunology, Shiraz University of Medical Sciences, Shiraz, Iran
Mehrnoosh
Doroudchi
Department of Immunology, Shiraz University of Medical Sciences, Shiraz, Iran
0000-0003-4067-5400
Bahia
Namavar Jahromi
2Infertility Research Center
Department of Obstetrics and Gynecology,
Shiraz University of Medical Sciences, Shiraz, Iran
Behrouz
Gharesi-Fard
Department of Immunology
Infertility Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
0000-0002-0162-3395
Journal Article
2018
09
24
Background: Polycystic ovary syndrome (PCOS) is considered as the most common cause of female infertility that affects 4-10% of women in the reproductive age. Previous studies have shown the role of a balanced immune response in a successful pregnancy and fertility. Objective: To investigate the T helper cells type 1 (Th1) /Th2/Th17/Treg paradigms in peripheral blood of infertile PCOS compared with normal fertile women. Methods: Peripheral blood mononuclear cells (PBMCs) were isolated at the late follicular phase from 10 PCOS and 10 fertile women. PBMCs were stimulated with PMA and ionomycin in the presence of Berefeldin A as Golgi stop agent to detect intracellular cytokine production (IFN-γ, IL-17, and IL-4) from CD3+CD4+T cells population indicating T helper (Th) cells subsets by flowcytometry. Moreover, regulatory T cells were enumerated using CD25 and Foxp3 markers. Results: In this study, we report that the frequency of Th1 cells was increased compared to Th2 cells in infertile PCOS when considering Th1/Th2 ratio (P=0.05). Analysis of Th17/Th2 ratio showed a significant difference with a bias toward Th17 dominancy in PCOS (P=0.02). The proportion of CD4+CD25+Foxp3+ regulatory T cells was significantly lower in PCOS patients than that of healthy fertile women (P=0.02). Conclusion: In summary, Th1 and Th17 bias and reduction of Treg and Th2 cells as regulators of immune responses might be involved in the pathogenesis of PCOS. These results are suggestive of an altered immune response to inflammatory status in PCOS patients, likely causing some complications such as infertility in these patients.
Shiraz Institute for Cancer Research
Iranian Journal of Immunology
1735-1383
15
3
2018
09
01
Vitamin D3 Induced Decrease in IL-17 and Malondialdehyde, and Increase in IL-10 and Total Antioxidant Capacity Levels in Patients with Irritable Bowel Syndrome
186
196
39388
10.22034/iji.2018.39388
EN
Reza
Amani
Diabetes Research Center, Health Research Institute, Department of Nutrition, Ahvaz Jundishapur
University of Medical Sciences, Ahvaz
Food Security Research Center, Health Research Institute,
Department of Clinical Nutrition, School of Nutrition and Food Science, Isfahan University of Medical
Sciences, Isfahan,
0000-0002-0074-4080
Amir
Abbasnezhad
Nutritional Health Research Center, Department of Nutrition, Lorestan University of
Medical Sciences, Khorramabad,
Nutrition and Metabolic Diseases Researcher Center, Department of
Nutrition, Ahvaz Jundishapur University of Medical Sciences,
0000-0002-9436-7334
Eskandar
Hajiani
Research Center for Infectious Diseases of
the Digestive System, Ahvaz Jundishapur University of Medical Sciences,
Bahman
Cheraghian
Research Center for Infectious
Diseases of Digestive System, Department of Biostatistics and Epidemiology, School of Public Health
Zahra
Abdoli
Department of Immunology, Faculty of medicine, Ahvaz Jundishapur University of Medical Sciences,
Ahvaz, Iran
Razieh
Choghakhori
Food Security Research Center, Health Research Institute,
Department of Clinical Nutrition, School of Nutrition and Food Science, Isfahan University of Medical
Sciences, Isfahan,
Journal Article
2018
09
24
Background: Given the variations in clinical presentation and physiopathological mechanisms in irritable bowel syndrome (IBS) subtypes, it is an acknowledged fact that the response to treatments can be disparate. Objective: To assess the effect of vitamin D on inflammatory cytokines (IL-17, IL-10, TNF-α), and biomarkers of oxidative stress (total antioxidant capacity (TAC), and malondialdehyde (MDA)) among IBS patients. Methods: A double-blind, randomized, placebo-controlled 6-month intervention study was carried out on 90 IBS patients (85 were analyzed), as defined by the Rome III criteria. Study participants were randomly assigned to receive either 50,000 IU vitamin D3 or a placebo fortnightly. Results: Vitamin D supplementation significantly reduced the IL-17 and MDA serum levels (P<0.05) and observably increased the TAC and IL-10 serum levels (P<0.05), compared with the placebo group. Comparing different bowel habit subtypes, we observed that it was only in diarrhea predominant IBS (IBS-D) that vitamin D supplementation was able to significantly reduce the serum levels of TNF-α and IL-17 (P<0.05). However, in all subtypes, IL-10 and TAC increased, while MDA decreased (P<0.05) in vitamin D group, compared to the placebo group. Conclusion: Vitamin D3 supplementation reduces the serum IL-17 and MDA levels, and augments the serum IL-10 and TAC levels in IBS patients, particularly in IBS-D subtype. Thus, the present study demonstrates the beneficial effects of vitamin D on patients with IBS-D.
Shiraz Institute for Cancer Research
Iranian Journal of Immunology
1735-1383
15
3
2018
09
01
Lower Frequency of HLA-DRB1*01 in Southwestern Iranian Patients with Atherosclerosis
197
206
39389
10.22034/iji.2018.39389
EN
Hossein
Golmoghaddam
Department of Immunology, School of Medicine, Shiraz University of Medical
Sciences, Shiraz, Iran
0000-0000-0000-0000
Shirin
Farjadian
Department of Immunology, School of Medicine, Shiraz University of Medical
Sciences, Shiraz, Iran
Shahdad
Khosropanah
Department of Cardiology, School of Medicine, Shiraz University of Medical
Sciences, Shiraz, Iran
Pooyan
Dehghani
Department of Cardiology, School of Medicine, Shiraz University of Medical
Sciences, Shiraz, Iran
Mehrnoosh
Doroudchi
Department of Immunology, School of Medicine, Shiraz University of Medical
Sciences, Shiraz, Iran
0000-0003-4067-5400
Journal Article
2018
09
24
Background: Human leukocyte antigen (HLA) complex is a gene family involved in antigen presentation associated with protection or susceptibility to inflammatory, infectious and autoimmune diseases. Atherosclerosis is a chronic inflammatory disease in which HLA molecules play a role in the initiation and development of the disease through presentation of self or foreign antigens to T cells. Objective: To investigate the association of HLA-DRB1 alleles with atherosclerosis in a sample of southwestern Iranians. Methods: We performed an analytical cross-sectional study involving 96 patients with atherosclerosis and 72 controls. HLA-DRB1 genotyping was performed by PCR-SSP method. Results: We observed a significantly lower frequency of DRB1*01 in patients with coronary artery atherosclerosis than in controls (4.68% vs. 13.1, P=0.0052, OR=3.09, CI 95%: 1.35-7.05). However, this allele showed a positive association with high blood pressure (P=0.009) in patients. Furthermore, DRB1*16 allele was associated with hyperlipidemia (P=0.008) in patients. Conclusion: Our results demonstrated that DRB1*01 may be a protective allele against atherosclerosis in individuals who live in southwest of Iran. The mechanism of this protection needs further investigation.
Shiraz Institute for Cancer Research
Iranian Journal of Immunology
1735-1383
15
3
2018
09
01
Designing and Expression of Recombinant Chimeric Protein Containing CtxB and OmpW from Vibrio Cholerae and Evaluation of Its Immunogenicity
207
220
39390
10.22034/iji.2018.39390
EN
Atina
Vakili
Department of Biology, Faculty of Basic Science, Shahed University, Tehran, Iran
Seyed Latif
Mousavi Gargari
Department of Biology, Shahed University, Tehran, Iran
0000-0002-4635-8716
Shahram
Nazarian
Department of Biology, Faculty of Science, Imam Hussein University, Tehran, Iran
Jafar
Amani
Applied Microbiology
Research center, System biology and poisonings institute, Baqiyatallah University of Medical Sciences,
Tehran, Iran
0000-0002-5155-4738
Journal Article
2018
09
24
Background: Cholera disease caused by Vibrio cholerae remains a major cause of morbidity and mortality throughout the world. Various strategies with different proteins as immunogens have been tried for vaccine development, none of which have been sufficiently effective to preclude cholera. Chimeric proteins, with their ability to present multiple antigens at the same time, can play important roles in immunization. Objective: To evaluate the immunogenicity of a chimeric construct, comprised of OmpW and CtxB as immunogenic proteins of Vibrio cholera, in BALB/c mice. Methods: The construct was designed after bioinformatics assessments and then expressed in E.coli. Chimeric protein, OmpW, and CtxB were purified with Ni-NTA chromatography and confirmed by Western blotting. Mice were immunized with purified recombinant proteins. The antibody titers and specificity of the immune sera were then analyzed by ELISA and challenged on the pups of immunized mice with 1, 5 and 10 LD50. Mice ileal loop assay was also performed. Results: Significant differences were observed in antibody titers in immunized mice compared to the control groups. Infant mouse challenge was performed so as to compare the protective efficacies of the selected immunogen regimens. Of the Pups from dams immunized with chimeric protein which received 1 LD50, 75% survived. Pups belonging to PBS-immunized dams, experienced 100% mortality. The serum raised toward immunogenic construct, inhibited cholera toxin activity in ileal loop test up to 68%. Conclusion: Chimeric construct is able to induce the immune system and provide up to 75% inhibition of toxin activity against 1 LD50 of Vibrio cholerae.
Shiraz Institute for Cancer Research
Iranian Journal of Immunology
1735-1383
15
3
2018
09
01
Circulating Levels of Interleukin-10 and -17 in Patients with Cerebral Sinovenous Thrombosis (CSVT) in Acute and Subacute Stages: A Prospective Case-Control Study
221
227
39391
10.22034/iji.2018.39391
EN
Sadegh
Izadi
Clinical Neurology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran,
Afshin
Borhani-Haghighi
Clinical Neurology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran,
0000-0002-4131-7990
Kamal
Bastani
Clinical Neurology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran,
Bahareh
Kardeh
Clinical Neurology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran,
Golnaz
Yadollahi-Khales
University of
Illinois hospital, neurology department, Chicago, Illinois
Mojtaba
Neydavoodi
Clinical Neurology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran,
Journal Article
2018
09
24
Background: Cerebral sinovenous thrombosis (CSVT) is a neurovascular disorder that occurs when a blood clot develops in a vein near the brain. Evaluating the subsequent changes in inflammatory cytokines can better reveal the underlying pathogeneses. Objective: To assess the serum levels of interleukin-10 (an anti-inflammatory cytokine) and IL-17 (a pro-inflammatory cytokine) in patients with aseptic non-vasculitic CSVT. Methods: In this prospective case-control study, 31 patients with aseptic non-vasculitic CSVT (admitted in Namazi Hospital, Shiraz University of Medical Sciences, Shiraz, Iran) were enrolled. IL-10 and IL-17 serum levels were measured at diagnosis, before initiation of treatment (acute stage), 3 months later (subacute stage). These cytokines were also measured in samples obtained from 30 gender- and age-matched healthy subjects, which were considered as control values. Results: Patients’ IL-10 and IL-17 levels were higher in both acute and subacute stages as compared to controls. However, no significant differences existed between the acute stage and control groups for both cytokines. Moreover, subacute levels were significantly higher than their acute and control levels. Conclusion: This study demonstrated the alteration of IL-10 and IL-17 levels in aseptic non-vasculitic CSVT. The rise in subacute IL-10 can be explained by the assumption that IL-10 is released as an anti-inflammatory response to subside the effects of IL-17 mediated reactions. More importantly, the immediate sampling in the acute stage did not allow enough time for triggering the immune system to produce such mediators. However, a balance was established between IL-10 and IL-17 in the subacute stage to prevent further tissue damage.
Shiraz Institute for Cancer Research
Iranian Journal of Immunology
1735-1383
15
3
2018
09
01
The Soluble and Particulate Form of Alginates Positively Regulate Immune Response
228
238
39392
10.22034/iji.2018.39392
EN
Fei
Ge
School of Biochemical Engineering, Anhui Polytechnic University, Wuhu, Anhui, China
Longbao
Zhu
School of Biochemical Engineering, Anhui Polytechnic University, Wuhu, Anhui, China
Liangjun
Yang
School of Biochemical Engineering, Anhui Polytechnic University, Wuhu, Anhui, China
Wanzhen
Li
School of Biochemical Engineering, Anhui Polytechnic University, Wuhu, Anhui, China
Shenghua
Wei
School of Biochemical Engineering, Anhui Polytechnic University, Wuhu, Anhui, China
Yugui
Tao
School of Biochemical Engineering, Anhui Polytechnic University, Wuhu, Anhui, China
Guocheng
Du
School of Biochemical Engineering, Anhui Polytechnic University, Wuhu, Anhui,
Key Laboratory of
Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, China
Journal Article
2018
09
24
Background: Alginate materials have been widely employed for biomedical applications ranging from wound healing to cancer treatment. However, how alginate materials affect the immune system is largely unknown. Objective: To explore the impact of alginate materials on immune system. Methods: The effect of three types of alginate materials, low viscosity, high viscosity and particulate alginate, were examined by both in vivo and in vitro analyses. C57BL/6J (B6) mice were treated with alginate and peripheral blood was tested by ELISA for cytokine production. Dendritic cells, macrophages and splenocytes isolated from mice were analyzed for the response to alginate treatment. Administration of alginates by intra lymph node injection (I.L.N.) yielded more potent cytokines productions than other injection routes. Results: Alginate materials did not affect the viability of lymphocytes. Particulate alginate induced the most potent inflammatory reaction as determined by the production of cytokines, such as, IL-1β, IL-8, TNF-α and IFN-γ. Low viscosity and particulate alginates are more effective than high viscosity alginates in activating dendritic cells as indicated by the expression of dendritic cells surface markers (CD80, CD86 and CD40). Similarly, the level of G-CSF was slightly higher in particulate alginate treated macrophages. Conclusion: Alginate materials could affect immune response through different ways, including promoting inflammatory cytokine production, and activating dendritic cells. Therefore, alginate materials, especially in particulate form, have the potential to be applied in inflammation related diseases.
Shiraz Institute for Cancer Research
Iranian Journal of Immunology
1735-1383
15
3
2018
09
01
Soluble CD163 Levels and CD163+CD14+ Monocyte/Macrophage Counts in Patients with Asthma
239
245
39393
10.22034/iji.2018.39393
EN
Yue
Zhi
Department of Respiratory and Critical Care Medicine, The Second Affiliated Hospital of Jilin University,
Department of Respiratory, Changchun Center Hospital, Changchun, Jilin, China
0000-0001-9663-6879
Peng
Gao
Department of Respiratory and Critical Care Medicine, The Second Affiliated Hospital of Jilin University, Changchun, Jilin, China
Wei
Li
Department of Respiratory and Critical Care Medicine, The Second Affiliated Hospital of Jilin University, Changchun, Jilin, China
Jie
Zhang
Department of Respiratory and Critical Care Medicine, The Second Affiliated Hospital of Jilin University, Changchun, Jilin, China
Fengling
Gao
Department of Respiratory, Changchun Center Hospital, Changchun, Jilin, China
Jichun
Zhang
Department of Respiratory, Changchun Center Hospital, Changchun, Jilin, China
Haitao
Lin
Department of Respiratory, Changchun Center Hospital, Changchun, Jilin, China
Journal Article
2018
09
24
Background: CD163-expressing macrophages are involved in the inflammatory response in asthma. Objective: To assess sputum and serum soluble CD163 (sCD163) and cytokine levels in patients with asthma. Further discussed was the difference between sCD163 and other classic inflammatory mediators. Methods: Sputum was successfully induced in asthma patients (n=85) and healthy controls (n=21). Interleukin (IL)-4, IL-5, IL-1β, IL-8, IL-9, IL-6, and sCD163 levels in sputum were measured. CD163+ monocytes in blood were evaluated using flow cytometry. Results: Sputum sCD163 level significantly increased in asthma (median: 22.4 pg/ml; IQR, 11.52-42.91), unlike healthy controls (10.54 pg/ml;9.85-23.5; P<0.001). Sputum sCD163 (P=0.020) and serum sCD163 (P=0.032) levels were significantly higher in patients with severe asthma compared to those with mild/moderate asthma. Percentage of CD163+ monocytes in patients with asthma was significantly lower than the controls (P<0.001). Conclusion: Increased sCD163 levels in sputum are associated with the impairment of lung function.