Mahendra Narain Mishra; Puja Dudeja; Rakesh Kumar Gupta
Volume 11, Issue 1 , March 2014, , Pages 21-28
Abstract
Background: Pediatric bronchial asthma is associated with considerable morbidity. The study was carried out to examine the association of Human Leukocyte Antigen (HLA)- Class II with the disease as we found no similar study on Asian Indian population. Objective: To define the HLA-Class II antigens in ...
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Background: Pediatric bronchial asthma is associated with considerable morbidity. The study was carried out to examine the association of Human Leukocyte Antigen (HLA)- Class II with the disease as we found no similar study on Asian Indian population. Objective: To define the HLA-Class II antigens in Asian Indian pediatric patients with asthma. Methods: A total of 103 children with asthma and 152 controls were analysed for HLA Class II (DRB1, DQB1and DPB1) by PCR-SSP (Sequence Specific Primers) method. Total serum IgE levels were determined by ELISA assay. Results: A positive family history was recorded in 59 patients (57%) and 13 (8.5%) of healthy controls. Serum IgE levels were more than normal range in 72% of the patients and 33% of healthy subjects with mean values of 4877 and 627 IU/ml, respectively. DRB1*04 and DQB1*03 showed significant positive relations while DRB1*15 showed a negative association with asthma. DQB1*02 was more common in healthy individuals but was not statistically significant. Conclusions: A positive association of the DR4/DQB1*03 and a negative association of DRB1*15 was seen with extrinsic bronchial asthma. However, more studies are required on larger populations to confirm the association of HLA Class II alleles in Indians before a particular allele can be labeled as being protective or causative for asthma.
Mahendra Narain Mishra; Rakesh Pandey; Ashok Dinda; Soniya Nityanand
Volume 10, Issue 3 , September 2013, , Pages 186-189
Mahendra Narain Mishra; Vinay Singai
Volume 7, Issue 4 , December 2010, , Pages 252-256
Abstract
Background: Spondyloarthropathies are a group of closely related inflammatory arthritis which involve the axial skeleton and are negative for rheumatoid factor. Objective: This case-control study was conducted to examine HLA- B27 positivity in patients with seronegative spondyloarthritis (SSA) as per ...
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Background: Spondyloarthropathies are a group of closely related inflammatory arthritis which involve the axial skeleton and are negative for rheumatoid factor. Objective: This case-control study was conducted to examine HLA- B27 positivity in patients with seronegative spondyloarthritis (SSA) as per ESSG criteria and compare the frequency with healthy controls because a lower positivity is reported in Indians. Method: The study included 453 patients and 200 controls. HLA -B27 typing was done by microlymphocytoxicity and/or by sequence specific primers (SSP) using commercial kits. Patients were categorised as Ankylosing Spondylitis (AS), Undifferentiated Spondyloarthropathy, SSA with inflammatory bowel disease, reactive arthritis, psoriatic arthritis and juvenile spondyloarthropathy. Results: HLA-B27 antigen was present in 56% of patient and 3.5% controls with highest frequency in juvenile spondyloarthropathy (80%), followed by AS (76%). The P value < 0.001 for all categories of SSA and overall Odds ratio was 34.9. Conclusion: This study showed HLA-B27 frequency slightly lower than reported in Caucasian SSA patients and in our opinion HLA- B27 testing is extremely useful in young patients with suspected SSA.