Mohammad Reza Ataollahi; Elham Aflaki; Mohammad Ali Nazarinia; Saeedeh Shenavandeh; Zahra Habibagahi; Behrouz Gharesi-Fard; Eskandar Kamali-Sarvestani
Volume 9, Issue 4 , December 2012, , Pages 241-247
Abstract
Background: The prevalence of anti-Neutrophil Cytoplasmic Antibodies (ANCAs) and anti-Cardiolipin Antibodies (anti-CL Ab) in Behcet’s Disease (BD) and also their roles in vascular involvement is controversial. Objective: To assess the prevalence of ANCAs and anti-CL Ab as well as their correlations ...
Read More
Background: The prevalence of anti-Neutrophil Cytoplasmic Antibodies (ANCAs) and anti-Cardiolipin Antibodies (anti-CL Ab) in Behcet’s Disease (BD) and also their roles in vascular involvement is controversial. Objective: To assess the prevalence of ANCAs and anti-CL Ab as well as their correlations with clinical manifestations in Iranian patients with BD. Methods: In this case/control study, the sera from 88 patients with BD and 88 healthy controls were evaluated. The levels of ANCAs and anti-CL Ab were measured using indirect ELISA method. Results: The levels of anti-CL, anti-PR3 and anti-MPO (Myeloperoxidase) IgG autoantibodies between BD patients and healthy controls were not statistically different (p=0.21, p=0.28 and p=0.74, respectively). In addition, there were no significant deferences between BD patients with and without vascular involvement in the levels of anti-CL (1.42 ± 1.24 GPLU/ml and 1.58 ± 1.18 GPLU/ml, respectively; p=0.71), anti-PR3 (0.0 ± 0.0 U/ml and 0.08 ± 0.27 U/ml, respectively; p=0.10) and anti MPO (0.48 ± 0.23 U/ml and 0.52 ± 0.22 U/ml, respectively; p=0.41) IgG autoantibodies. Nevertheless, mean titer of anti-CL IgG was higher in male patients with skin rash than those without skin rash (2.2 ± 0.88 GPLU/ml and 1.11 ± 1.22 GPLU/ml, respectively; p=0.017). Conclusion: While anti-CL, anti- PR3 and anti-MPO IgG autoantibodies do not play a major role in susceptibility to BD or pathogenesis of vascular involvement in our patients, anti-CL Ab might be involved in skin lesion development in Iranian male BD patients. However, the results should be confirmed in other studies.
Zahra Habibagahi; Mohammad Ali Nazarinia; Elham Aflaki; Akbar Rajaee
Volume 4, Issue 3 , December 2007, , Pages 161-166
Abstract
Background: The clinical significance of antiphospholipid antibodies in patients with chronic hepatitis C virus (HCV) and some other viral infections is controversial. Objective: To study the prevalence of anticardiolipin antibody (ACLA) and antibeta2glycoproteinI antibody (antibeta2GPI antibody) in ...
Read More
Background: The clinical significance of antiphospholipid antibodies in patients with chronic hepatitis C virus (HCV) and some other viral infections is controversial. Objective: To study the prevalence of anticardiolipin antibody (ACLA) and antibeta2glycoproteinI antibody (antibeta2GPI antibody) in HCV and hepatitis B virus (HBV) infected patients and its association with liver clinical parameters. Methods: Serum levels of ACLA, antibeta2GPI antibody as well as platelet count, ALT (alanine transaminase), PT (prothrombine time), disease duration and liver histologic findings of 38 patients with HBV and 15 patients with HCV infections were compared with those of 58 healthy controls. Results: Serum titres of ACLA in HCV and HBV patients (13.4 ±7.1 GPL units/ml), and in each of the HCV (15.18±9.91 GPL units/ml) and HBV (12.7 ± 5.7 GPL units/ml) patients were significantly higher than that of the control group (3.4±2.3GPL units/ml). However, there was no significant difference in serum levels of antibeta2GPI antibody from patients with HCV and HBV (3.3 ± 1.3 GPL units/ml) or HCV alone (2.79 ± 1.01 GPL units/ml) or HBV alone (3.4±1.3GPL units/ml) and that of the control group (3.3±1.1GPL units/ml). Conclusion: The findings suggest that the presence of ACLA has no pathologic significance in patients with HBV and HCV infections.
Akbar Rajaee; Mohammad Ali Nazarinia; Seyed Modjtaba Hakim; Mitra Amini; Maryam Ayatollahi; Abbas Ghaderi
Volume 1, Issue 2 , September 2004, , Pages 111-117
Abstract
Objective: The clinical value of IgG anticardiolipin antibody in patients with Behçet's disease with or without vascular thrombosis was evaluated. Methods: IgG isotype of anticardiolipin (aCL) antibody was assessed in 40 Behçet's disease (BD) patients with venous or arterial thrombosis, ...
Read More
Objective: The clinical value of IgG anticardiolipin antibody in patients with Behçet's disease with or without vascular thrombosis was evaluated. Methods: IgG isotype of anticardiolipin (aCL) antibody was assessed in 40 Behçet's disease (BD) patients with venous or arterial thrombosis, 40 BD patients without venous or arterial thrombosis and 80 healthy subjects as controls. The levels of IgG aCL were determined by an indirect ELISA method. Color Doppler Sonography, Magnetic Resonance Imaging and conventional angiography were the procedures used for other clinical evaluations. Results: Out of 40 patients with vascular thrombosis, 20(50%) were positive for low to moderate level of IgG aCL. In patients without thrombosis 22(55%) were positive for low to moderate level of IgG aCL while in none (0%) of the healthy subjects the IgG aCL was positive, neither low nor moderate. The number of patients with headache but having a normal cerebral magnetic resonance imaging (MRI), was higher in anticardiolipin positive patients without vascular thrombosis as compared to those with vascular thrombosis, (P = 0.001). Arthritis was noticed in both patents groups. 15% of aCL positive patients without thrombosis had arthritis as compared to none in aCL negative patients without thrombosis (P = 0.02). Conclusion: The results of this study indicate that although the frequency of IgG aCL was found to be higher in Iranian patients with BD in comparison with the previous reports, except in arthritis the observed elevated IgG aCL does not correlate with clinical disease manifestations, or vascular thrombotic complications.