Marco Di Tola; Mariacatia Marino; Rossella Casale; Marta Puzzono; Caterina Urciuoli; Antonio Picarelli
Abstract
Background: Celiac disease is a common autoimmune disorder that is diagnosed based on clinical case identification, serological screening, and duodenal histology. However, the existence of mild clinical forms, such as seronegative cases with patchy atrophy and potential celiac disease, can make it difficult ...
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Background: Celiac disease is a common autoimmune disorder that is diagnosed based on clinical case identification, serological screening, and duodenal histology. However, the existence of mild clinical forms, such as seronegative cases with patchy atrophy and potential celiac disease, can make it difficult to determine a definitive diagnosis. The seronegative patients with celiac disease can include those with discordant antibody results and false-negative results, due to unknown origins or selective IgA deficiency. Case presentation: We present two cases with discordant antibody results in order to evaluate if the simultaneous detection of specific antibodies can improve the serodiagnosis of celiac disease. In both patients, the simultaneous detection of IgA/IgG anti-tissue transglutaminase/deamidated gliadin peptides gave discordant positive results by the same antibodies assayed individually. Conclusion: Although further studies are needed to confirm and extend our findings, the simultaneous detection of specific antibodies seems to improve the serodiagnosis of celiac disease in patients with discordant antibody results.
Seyed Mahmoud Sadjjadi; Hassan Abidi; Bahador Sarkari; Ahmad Izadpanah; Sakineh Kazemian
Abstract
Background: Hydatidosis is one of the cosmopolitan parasitic zoonoses caused by the larval stage of Echinococcus granulosus. Diagnosis of hydatidosis is still an unresolved problem. Serological tests using crude antigens for diagnosis of E. granulosus are sensi-tive, however their specificity are not ...
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Background: Hydatidosis is one of the cosmopolitan parasitic zoonoses caused by the larval stage of Echinococcus granulosus. Diagnosis of hydatidosis is still an unresolved problem. Serological tests using crude antigens for diagnosis of E. granulosus are sensi-tive, however their specificity are not satisfactory. Therefore, WHO recommended spe-cific serological methods using specific antigens, specially native AgB for proper diagno-sis. Objectives: This study was designed to evaluate the ELISA and counter current im-munoelectrophresis (CCIEP) method using native antigen B (Ag B) for serodiagnosis of human hydatidosis in Fars Province, Iran, an endemic area for this parasitic disease. Methods: Native AgB was purified from sheep hydatid fluid. Serum samples obtained from 40 pathologically confirmed cases of hydatidosis along with samples from patients with fascioliasis, toxocariasis, taeniasis and cancer patients and sera from healthy indi-viduals were tested by ELISA using native antigen B or tested by countercurrent immu-noelectrophresis (CCIEP) using crude sheep hydatid cyst fluid. Results: Sensitivity of the ELISA system was determined to be 92.5% and the specificity was found to be 97.3%. Positive and negative predictive values of the system were 92.5% and 97.3%, respec-tively. For countercurrent immunoelectrophresis the sensitivity of the assay was 97.5% and its specificity was 58.18%. This ELISA system is much more specific in detecting anti hydatid cyst antibody than CCIEP, while CCIEP is more sensitive in detecting anti hydatid cyst antibody. Conclusion: The new ELISA system using native antigen B is a suitable method and preferable to CCIEP for immunodiagnosis of human hydatidosis.
