Kazem Fatemi; Seyed Abdolrahim Rezaee; Seyed Ali Banihashemrad; Sanaz Keyvanfar; Meghdad Eslami
Abstract
Background: Matrix metalloproteinases (MMPs) stimulate alveolar bone loss in chronic periodontitis. Objective: To evaluate the salivary and gingival crevicular fluid (GCF) levels of MMP-8 in patients with moderate to severe chronic periodontitis. Methods: 42 participants were divided into two groups: ...
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Background: Matrix metalloproteinases (MMPs) stimulate alveolar bone loss in chronic periodontitis. Objective: To evaluate the salivary and gingival crevicular fluid (GCF) levels of MMP-8 in patients with moderate to severe chronic periodontitis. Methods: 42 participants were divided into two groups: a case group (21 patients with generalized moderate to severe chronic periodontitis) and a control group (21 healthy periodontal subjects). GCF and saliva samples were obtained from both groups. Salivary and GCF MMP-8 levels of each subject were detected using the ELISA method. Results: Mean±SD values of salivary MMP-8 levels of the control and case groups were 1.52 ± 0.65 ng/ml and 6.06 ± 1.18 ng/ml, respectively, and statistically significant difference was observed (p=0.0001). Also, mean±SD values of GCF MMP-8 levels of the control and case groups were 0.87 ± 0.26 ng/ml and 2.92 ± 0.64 ng/ml, respectively; which was statistically significant (p=0.0001). Conclusion: Our results demonstrate an increased concentration of salivary and GCF levels of MMP-8 in the patient group.
Beheshteh Malekafzali; Mandana Sattari; Sanaz Keyvanfar
Abstract
Background: Early childhood caries (ECC) is a common health problem in the developing countries. Basic knowledge about the etiology and pathogenesis of ECC plays an important role in its prevention. Objective: To determine the relationship between salivary TLR-2 concentration and early childhood caries ...
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Background: Early childhood caries (ECC) is a common health problem in the developing countries. Basic knowledge about the etiology and pathogenesis of ECC plays an important role in its prevention. Objective: To determine the relationship between salivary TLR-2 concentration and early childhood caries formation Methods: Twenty-Eight children with ages ranging from 36 to 71 months (15 in ECC group and 13 in caries free group) were chosen based on inclusion criteria. Their saliva was aspirated in the volumes of 1-2 ml. Resampling was done for 8 subjects of ECC group 3 months after dental restoration. TLR-2 concentration was measured using ELISA. Results: Mean concentrations of TLR-2 in ECC and caries free group were 2.12 and 1.42 ng/ml, respectively. The difference between concentrations was statistically significant (p=0.008). Three months after treatment in 8 ECC, the mean concentration of TLR-2 (0.925 ng/ml) significantly decreased compared to the original concentration in ECC (p<0.001) and caries free groups (p<0.001). Conclusion: Elevated concentration of TLR-2 in ECC group compared to caries free group and its decrease after treatment point to the participation of innate immune system and specially TLR-2 in the pathogenesis of early childhood caries.
Mina Biria; Mandana Sattari; Mojtaba Vahid Golpayegani; Fahimeh Kooshki
Abstract
Background: Early childhood caries (ECC) is a severe type of dental caries affecting infants and pre-school children. Because of the infectious nature of the disease, the immunologic response by the host plays an essential role in its development. Objective: This study investigated the association between ...
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Background: Early childhood caries (ECC) is a severe type of dental caries affecting infants and pre-school children. Because of the infectious nature of the disease, the immunologic response by the host plays an essential role in its development. Objective: This study investigated the association between the presence of salivary sCD14 and ECC. Methods: This study was carried out on 40 healthy children, of whom 20 were caries-free (CF) and 20 had ECC, within the ages of 36 to 71 months. Unstimulated saliva of the children was collected with disposable needle-less syringe from buccal and labial vestibules. Seven children with ECC received complete treatments. Saliva was collected for a second time after 3 months from this group. The sCD14 levels in salivary samples were analyzed by ELISA method. Results: Mean concentrations of sCD14 in ECC and CF groups were 57.82 and 31.92 ng/ml respectively (p=0.008). After three months, the mean concentration of sCD14 among the treated children decreased to 11.38 ng/ml, which was significantly lower compared to that of ECC children before intervention (p<0.001), and also CF children (p<0.05). Conclusion: The increased levels of sCD14 can be considered as a marker of inflammation and innate immune response during ECC.
Ali Bagherian; Abdullah Jafarzadeh; Mohsen Rezaeian; Shima Ahmadi; Mohammad Taghi Rezaity
Abstract
Background: Early Childhood Caries (ECC) is one of the most common chronic child-hood diseases. In spite of the global decrease in dental caries in the past decades, ECC has become a significant problem in many developing countries and also in a few indus-trialized nations. Saliva as a host factor can ...
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Background: Early Childhood Caries (ECC) is one of the most common chronic child-hood diseases. In spite of the global decrease in dental caries in the past decades, ECC has become a significant problem in many developing countries and also in a few indus-trialized nations. Saliva as a host factor can play an important role in the process of den-tal caries. Objective: The aim of this study was to compare sIgA and IgG as saliva components between ECC and caries-free groups. Methods: In this cross-sectional study, samples of unstimulated saliva of 90 children (45 in ECC group & 45 in caries-free group) were taken with Scully method. Then the concentration levels of sIgA and IgG were measured with Enzyme Linked Immunosorbent Assay and Single Radial Im-munodiffusion methods. Results: Mean concentration levels of salivary sIgA and IgG were significantly higher among children with ECC (p<0.05). There was also a weak inverse correlation between sIgA level and DMFT index in ECC group but it was not statistically significant (p=0.056). Conclusion: The high concentration of salivary im-munoglobulin in children with ECC may be associated with an increased antigenic load, leading to high production of antibodies.
