Ali Ahmadi; Najmeh Poursasan; Jafar Amani; Jafar Salimian
Volume 12, Issue 1 , March 2015, , Pages 64-69
Abstract
Background: T-2 mycotoxin belongs to the Trichothecene family and has damaging effects on the immune system. Objective: To investigate the toxic effect of T-2 toxin on the percentage of peripheral blood B lymphocytes and the potential protective role of selenium and vitamin E. Method: Frequencies of ...
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Background: T-2 mycotoxin belongs to the Trichothecene family and has damaging effects on the immune system. Objective: To investigate the toxic effect of T-2 toxin on the percentage of peripheral blood B lymphocytes and the potential protective role of selenium and vitamin E. Method: Frequencies of B lymphocytes (CD19+ ) were analyzed after injection of sublethal doses of T-2 toxin into Balb/c mice at different time points, using flowcytometry. Additionally, the effects of selenium and vitamin E on B lymphocyte, as either prophylaxis or simultaneously administered with T-2 toxin, were investigated. Results: After injection of a sublethal dose of T-2 toxin, the number of B cells (CD19+ ) significantly decreased at 12 h and became normal at 72 h. When selenium was injected both 24 h before and simultaneously with T-2 toxin, it was able to inhibit B lymphocyte (CD19+) reduction. In contrast, injecting vitamin E, 24 h before or simultaneously with T-2 toxin did not regulate B lymphocyte alteration. Conclusion: Selenium plays pivotal role on altered B lymphocyte subset induced by T-2 toxin comparing to vitamin E.
Kazem Ahmadi; Majid Riazipour
Volume 5, Issue 3 , September 2008, , Pages 177-180
Abstract
Background: T-2 toxin is a mycotoxin of type A trichothecenes produced by several fungal genera such as Fusarium species. Mycotoxins can affect both cell mediated and humoral immune compartments. Objective: The purpose of this study was to investi-gate the effect of T-2 toxin on cytokine production by ...
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Background: T-2 toxin is a mycotoxin of type A trichothecenes produced by several fungal genera such as Fusarium species. Mycotoxins can affect both cell mediated and humoral immune compartments. Objective: The purpose of this study was to investi-gate the effect of T-2 toxin on cytokine production by mouse peritoneal macrophages and lymph node T cells. Methods: Mouse peritoneal macrophages and lymph node T cells were isolated and treated with different concentrations of T-2 toxin and incubated at 370C and 5% CO2 in air for 48 hours. Cell free media were removed and used for cy-tokine assay by an ELISA method. Results: T-2 toxin significantly reduced IL-1β re-lease in a concentration dependent manner (p<0.005, p<0.001). Interleukin-12 and TNF-α production were significantly increased in response to 0.001ng/ml, 0.01ng/ml and 0.1ng/ml of T-2 toxin (p<0.001). However, T-2 toxin at higher concentrations rang-ing from 1ng/ml to 100ng/ml, reduced both IL-12 (p<0.001) and TNF-α production (p<0.005, p<0.05). The effects of T-2 toxin on lymph node T cells showed that IL-4 and IL-10 release was decreased in a concentration dependent manner (all with p<0.01). T-2 toxin at concentrations between 1ng/ml and 100ng/ml reduced the release of both IL-2 and IFN-γ (p<0.05, p<0.001). Conclusion: The results suggest that T-2 toxin at low concentrations can highly induce secretion of IL-12, TNF-α, IFN-γ and IL-2 and it may be used as a positive immunomodulator in the human model.