@article { author = {Khalili, Ahmad and Muhammad Hassan, Zuhair and Shahabi, Shahram and Pourfathollah, Ali Akbar and Ostad, Seyed Nasser and Noori, Shokoofe and Mahdavi, Mehdi and Haybar, Habib and Langroudi, Ladan}, title = {Long Acting Propranolol and HSP-70 Rich Tumor Lysate Reduce Tumor Growth and Enhance Immune Response against Fibrosarcoma in Balb/c Mice}, journal = {Iranian Journal of Immunology}, volume = {10}, number = {2}, pages = {70-82}, year = {2013}, publisher = {Shiraz Institute for Cancer Research}, issn = {1735-1383}, eissn = {1735-367X}, doi = {}, abstract = {Background: Noradrenaline (NA), the principal neurotransmitter released from sympathetic nerve terminals, influences T-cell maturation, not only directly in developing T cells, but also indirectly, by acting on the thymic nonlymphoid cells. In vitro and in vivo studies have demonstrated the anti-proliferative, anti-migratory, antiangiogenic and cytotoxic properties of propranolol, β-AR blocker, against various cancers. Objectives: To evaluate the effect of propranolol on efficacy of HSP-70 rich lysate vaccine in immunotherapy of fibrosarcoma. Methods: Mouse fibrosarcoma WEHI-164 cells were used to immunize tumor-bearing mice with or without propranolol and HSP-70. Splenocytes proliferation, cytotoxic activity of the splenocytes, naturally occurring CD4+ CD25high T-reg cells and IFN-γ and IL-4 secretion as well as tumor size, were assessed to describe the anti-tumor immune response. Results: A significant increase in the level of IFN-γ in the mice vaccinated with WEHI-164 cells enriched with HSP-70 and co-treated with propranolol was observed compared to controls. However, HSP enrichment or propranolol treatment alone did not enhance the immune response as measured by the level of IFN-γ. Likewise, a decrease in tumor growth in the test group (p<0.01) and a significant increase in CTL activity (p<0.05) was observed. Conclusion: HSP enriched vaccine shows anti-tumor activity, probably due to the modulation of immune responses.}, keywords = {IFN-γ,IL-4,Immune Modulator,Propranolol,T Regulatory Cell}, url = {https://iji.sums.ac.ir/article_16811.html}, eprint = {https://iji.sums.ac.ir/article_16811_8a945847531ec52d8ad794bd12bb912c.pdf} }