@article { author = {Forghani, Hossein and Jamshidi Makiani, Mahin and Zarei Jaliani, Hossein and Zahraei, Seyed Mohsen and Namayandeh, Seyedeh Mahdieh and Khani, Parisa}, title = {Toward an Alum Free Mono-Component Monovalent Pertussis Vaccine: A Cytokine Response Assay}, journal = {Iranian Journal of Immunology}, volume = {17}, number = {2}, pages = {111-120}, year = {2020}, publisher = {Shiraz Institute for Cancer Research}, issn = {1735-1383}, eissn = {1735-367X}, doi = {10.22034/iji.2020.86199.1749}, abstract = {Background: Currently evidence indicates the resurgence of whooping cough despite high coverage of whole-cell (wP) and acellular (aP) pertussis vaccines. Objective: In this study, we investigated the cell-mediated immune response of a genetically inactivated protein containing the S1 subunit of pertussis toxin (PTS1) without and with the Listeriolysin O (LLO-PTS1), developed by the researchers (the authors of this study), in comparison with current wP and aP vaccines in the mice model. Methods: Thirty-six female NMRI mice aged 8 to 12 weeks (25 ± 5 g) were divided into six groups including control (n=6), and five treated groups (n=6/each). Treated groups comprising recombinant PTS1, recombinant fusion LLO-PTS1, aP, wP, and sham (phosphate-buffered saline) were injected intraperitoneally whereas the control group did not receive anything. After 60 days, the serum levels of IFN-γ, IL-4, and IL-17 cytokines (as the T-helper 1, 2, and 17 responses, respectively) were evaluated by mouse ELISA Kit. Results: Our findings showed LLO-PTS1 significantly increased IL-17 and IL-4 cytokines compared with wP and aP vaccines (superiority). IFN-γ failed to significantly increase in the LLO-PTS1 group compared to others but it was non-inferior to standard vaccines (non-inferiority). Conclusion: Our alum free mono-component monovalent recombinant fusion protein (LLO-PTS1), registered as a patent in the www.iripo.ssaa.ir, could bear the capacity to stimulate the Th-1 response similar to wP and aP vaccines (non-inferiority) in the mice model. In addition, it showed better results in Th-17 and Th-2 response (superiority). This study can be regarded as a springboard for further probes in booster pertussis vaccine development.}, keywords = {Fusion Protein,Listeriolysin O,LLO-PTS1,PST1,Pertussis,Vaccine}, url = {https://iji.sums.ac.ir/article_46744.html}, eprint = {https://iji.sums.ac.ir/article_46744_04919b600e6ffeabc28541970a93a01a.pdf} }