%0 Journal Article %T Adverse Effect of T-2 Toxin and the Protective Role of Selenium and Vitamin E on Peripheral Blood B lymphocytes %J Iranian Journal of Immunology %I Shiraz Institute for Cancer Research %Z 1735-1383 %A Ahmadi, Ali %A Poursasan, Najmeh %A Amani, Jafar %A Salimian, Jafar %D 2015 %\ 03/01/2015 %V 12 %N 1 %P 64-69 %! Adverse Effect of T-2 Toxin and the Protective Role of Selenium and Vitamin E on Peripheral Blood B lymphocytes %K B Lymphocytes %K Flowcytometry %K Selenium %K T-2 toxin %K Vitamin E %R %X Background: T-2 mycotoxin belongs to the Trichothecene family and has damaging effects on the immune system. Objective: To investigate the toxic effect of T-2 toxin on the percentage of peripheral blood B lymphocytes and the potential protective role of selenium and vitamin E. Method: Frequencies of B lymphocytes (CD19+ ) were analyzed after injection of sublethal doses of T-2 toxin into Balb/c mice at different time points, using flowcytometry. Additionally, the effects of selenium and vitamin E on B lymphocyte, as either prophylaxis or simultaneously administered with T-2 toxin, were investigated. Results: After injection of a sublethal dose of T-2 toxin, the number of B cells (CD19+ ) significantly decreased at 12 h and became normal at 72 h. When selenium was injected both 24 h before and simultaneously with T-2 toxin, it was able to inhibit B lymphocyte (CD19+) reduction. In contrast, injecting vitamin E, 24 h before or simultaneously with T-2 toxin did not regulate B lymphocyte alteration. Conclusion: Selenium plays pivotal role on altered B lymphocyte subset induced by T-2 toxin comparing to vitamin E. %U https://iji.sums.ac.ir/article_16735_6c68e5eb77d2ba626ff4d677756a545d.pdf