%0 Journal Article %T Propylene-Glycol Aggravates LPS-Induced Sepsis through Production of TNF-α and IL-6 %J Iranian Journal of Immunology %I Shiraz Institute for Cancer Research %Z 1735-1383 %A Marton, Annamaria %A Kolozsi, Csongor %A Kusz, Erzsebet %A Olah, Zoltan %A Letoha, Tamas %A Vizler, Csaba %A Pecze, Laszlo %D 2014 %\ 06/01/2014 %V 11 %N 2 %P 113-122 %! Propylene-Glycol Aggravates LPS-Induced Sepsis through Production of TNF-α and IL-6 %K Inflammation %K NF-KB %K Propylene-Glycol %K Sepsis %R %X Background : Propylene glycol (1,2-propanediol, PG) is a commonly used solvent for oral, intravenous, as well as topical pharmaceutical preparations. While PG is generally considered to be safe, it has been known that large intravenous doses given over a short period of time can be toxic. Objective: To evaluate the effect of PG in sepsis induced by the bacterial endotoxin lipopolysaccharide (LPS). Methods: Balb/c mice were treated with LPS (1 mg/kg b.w., i.p.) with or without PG (5 g/kg b.w. i.v.). The survival rate and the production of inflammatory cytokines were measured. In RAW264.7 mouse macrophages encoding NF- B-luc reporter gene, the nuclear transcription factor kappa- B (NF- B) activation was measured. Results: We found that intravenous PG increased the mortality rate in sepsis induced by the bacterial endotoxin lipopolysaccharide (LPS) in mice. In accordance with that, PG enhanced LPS -induced production of inflammatory cytokines, including tumor necrosis factor-α (TNF -α) and interleukin-6 (IL -6) in vivo. PG also increased the LPS-induced macrophage activation in vitro as detected by measuring NF- B activation. Conclusion: Our results indicate that drugs containing high doses of PG can pose a risk when administered to patients suffering from or prone to Gram negative bacterial infection. %U https://iji.sums.ac.ir/article_16772_5a9c8861c95db295aed3b1c25429dfb4.pdf