%0 Journal Article %T Is CD19 an Immunological Diagnostic Marker for Acute Appendicitis? %J Iranian Journal of Immunology %I Shiraz Institute for Cancer Research %Z 1735-1383 %A Mosayebi, Ghasem %A Alizadeh, Shaban Ali %A Alasti, Ali %A Amouzandeh Nobaveh, Alireza %A Ghazavi, Ali %A Okhovat, Mahsa %A Rafiei, Mohammad %D 2013 %\ 12/01/2013 %V 10 %N 4 %P 216-228 %! Is CD19 an Immunological Diagnostic Marker for Acute Appendicitis? %K Acute Appendicitis %K CD19 %K HLA-DR %K Lymphocyte Subsets %K Proliferation %R %X Background: The appendix is considered as part of the gut-associated lymphoid tissue; however, lymphocyte subsets in this tissue are not fully defined. Objective: To investigate and compare the function and phenotype of lymphocyte subsets in peripheral blood and appendix of patients with normal and inflamed appendix tissues. Methods: Peripheral blood samples and appendiceal mononuclear cells were obtained from 81 patients (mean age; 23 ± 10.5 years), clinically suspected of having appendicitis. The phenotypic characteristics of lymphocyte subsets in peripheral blood (before and 48-72 hrs after appendectomy) and in appendix tissue were analyzed by three color-flow cytometry. The proliferative response of mononuclear cells was assessed by MTT method. Results: The frequency of CD19+DR+, HLA-DR+ and CD19+ cells in the appendix tissue were significantly higher than that of the peripheral blood in all the groups (p<0.001). The percentage of CD19+ cells and HLA-DR+CD19+ cells significantly decreased after appendectomy in the peripheral blood of the patients with acute appendicitis (p=0.047 and p=0.03, respectively). CD19 and HLA-DR plus CD19 had better diagnostic efficiency compared with T cell markers (area under the ROC curve [AUC]= 0.76 and 0.73, respectively). Conclusion: These results indicate a significant difference in CD19+ and HLA-DR+ lymphocytes between peripheral blood and the appendix tissue. %U https://iji.sums.ac.ir/article_16838_164b8a85065e379e6b9d9745de4b5d2b.pdf