%0 Journal Article %T TLR9/NF-kB Pathway Regulates Brucella CpG DNA-mediated Cytokine Response in Human Peripheral Blood Mononuclear Cells %J Iranian Journal of Immunology %I Shiraz Institute for Cancer Research %Z 1735-1383 %A Peng, Yunzhi %A Bai, Wenhui %A Wang, Zhanli %A Yu, Hui %D 2021 %\ 12/01/2021 %V 18 %N 4 %P 268-278 %! TLR9/NF-kB Pathway Regulates Brucella CpG DNA-mediated Cytokine Response in Human Peripheral Blood Mononuclear Cells %K Agonist %K Brucella %K Cytidine-phosphateguanosine (CpG) motif %K Toll-like receptor 9 %R 10.22034/iji.2021.84578.1665 %X Background: It was reported that targeting the Toll-like receptor9(TLR9) signaling pathway can be a promising therapeuticstrategy forinterventions in various inflammatory and infectiousdiseases. However,it was not known whether the human TLR9 isresponsive to Brucellacytidine-phosphate-guanosine (CpG) DNAsequences and activatesthehost’s innate immune system. Objective: The present study aimed to identify the novel humanTLR9agonists from Brucella CpG oligodeoxynucleotide(ODN) candidatesand verify their immune response regulatorymechanisms. Methods: Molecular docking methods were used to discover potentagonists of the human TLR9. The potential molecules were furthervalidated by Western blot and enzyme-linked immunosorbentassay(ELISA). Results: The experiment results showed a strong interactionandgood compatibility between the human TLR9 and BrucellaODN-1molecule. In addition, the induction of immune response byBrucella ODN-1 is a CpG-specific response. Moreover, the effectsof Brucella ODN-1 on cytokine response are dependent on theTLR9-mediated NF-κB pathway. Conclusion: These results indicated that the Brucella ODN-1 molecule canserve as a starting point to discover or designmore potent and specific TLR9 agonists that have the potential usein the treatment of infectious diseases. %U https://iji.sums.ac.ir/article_48025_2103fd0547a484fed4b2ff97070092d9.pdf