TY - JOUR ID - 16809 TI - Detection of IL-20R1 and IL-20R2 mRNA in C57BL/6 Mice Astroglial Cells and Brain Cortex Following LPS Stimulation JO - Iranian Journal of Immunology JA - IJI LA - en SN - 1735-1383 AU - Abd Nikfarjam, Bahareh AU - Ebtekar, Massoumeh AU - Sabouni, Farzaneh AU - Pourpak, Zahra AU - Kheirandish, Maryam AD - Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University AD - National Institute of Genetic Engineering and Biotechnology AD - Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences and Health Services AD - Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran Y1 - 2013 PY - 2013 VL - 10 IS - 2 SP - 62 EP - 69 KW - Astroglial Cells KW - IL-20R1 KW - IL-20R2 KW - LPS KW - mice DO - N2 - Background: Astrocytes, which comprise ~90% of overall brain mass, are involved in brain immunity. These cells represent the non-professional class of CNS-resident APCs and may promote or inhibit CNS inflammation depending on the cytokines they secrete. IL-10 family of cytokines and their receptors, IL-20R1 and IL-20R2, may have a role in shifting astrocytes to a neuroprotective or neurodegenerative function. Objective: To address the expression of IL-20R1 and IL-20R2 cytokine receptors in astrocytes and brain cortex of C57BL/6 mice. Methods: We investigated the expression of IL-20R1 and IL-20R2 in C57BL/6 mice astroglial cells and brain cortex in response to lipopolysaccharide (LPS), using reverse-transcription polymerase chain reaction (RTPCR) method. Results: Astrocytes were able to express IL-20R1 and IL-20R2 mRNA not only in response to LPS stimulation but also in the absence of LPS. Furthermore, we found the expression of IL-20R1 and IL-20R2 mRNA in the cortex of adult C57BL/6 mice. Conclusions: IL-20R1 and IL-20R2 are constitutively expressed in the brain. Since most neuropathological processes involve astrocytes and inflammatory cytokines, these findings have important implications for future therapeutic strategies. UR - https://iji.sums.ac.ir/article_16809.html L1 - https://iji.sums.ac.ir/article_16809_d4ca233c60c0b6c779d7564d3bdad5fb.pdf ER -