TY - JOUR ID - 16811 TI - Long Acting Propranolol and HSP-70 Rich Tumor Lysate Reduce Tumor Growth and Enhance Immune Response against Fibrosarcoma in Balb/c Mice JO - Iranian Journal of Immunology JA - IJI LA - en SN - 1735-1383 AU - Khalili, Ahmad AU - Muhammad Hassan, Zuhair AU - Shahabi, Shahram AU - Pourfathollah, Ali Akbar AU - Ostad, Seyed Nasser AU - Noori, Shokoofe AU - Mahdavi, Mehdi AU - Haybar, Habib AU - Langroudi, Ladan AD - Department of Immunology, School of Medical Sciences, Tarbiat Modares University, Tehran AD - Department of Immunology, Microbiology and Genetics, Faculty of Medicine, Urmia University of Medical Sciences, Urmia AD - Department of Toxicology and Pharmacology Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran AD - Department of Analytical Chemistry, College of Sciences, Shahid Beheshti University AD - Department of Virology, Pasteur Institute of Iran, Tehran AD - Department of Anatomy, Ahwaz University of Medical Science, Ahwaz, Iran Y1 - 2013 PY - 2013 VL - 10 IS - 2 SP - 70 EP - 82 KW - IFN-γ KW - IL-4 KW - Immune Modulator KW - Propranolol KW - T Regulatory Cell DO - N2 - Background: Noradrenaline (NA), the principal neurotransmitter released from sympathetic nerve terminals, influences T-cell maturation, not only directly in developing T cells, but also indirectly, by acting on the thymic nonlymphoid cells. In vitro and in vivo studies have demonstrated the anti-proliferative, anti-migratory, antiangiogenic and cytotoxic properties of propranolol, β-AR blocker, against various cancers. Objectives: To evaluate the effect of propranolol on efficacy of HSP-70 rich lysate vaccine in immunotherapy of fibrosarcoma. Methods: Mouse fibrosarcoma WEHI-164 cells were used to immunize tumor-bearing mice with or without propranolol and HSP-70. Splenocytes proliferation, cytotoxic activity of the splenocytes, naturally occurring CD4+ CD25high T-reg cells and IFN-γ and IL-4 secretion as well as tumor size, were assessed to describe the anti-tumor immune response. Results: A significant increase in the level of IFN-γ in the mice vaccinated with WEHI-164 cells enriched with HSP-70 and co-treated with propranolol was observed compared to controls. However, HSP enrichment or propranolol treatment alone did not enhance the immune response as measured by the level of IFN-γ. Likewise, a decrease in tumor growth in the test group (p<0.01) and a significant increase in CTL activity (p<0.05) was observed. Conclusion: HSP enriched vaccine shows anti-tumor activity, probably due to the modulation of immune responses. UR - https://iji.sums.ac.ir/article_16811.html L1 - https://iji.sums.ac.ir/article_16811_8a945847531ec52d8ad794bd12bb912c.pdf ER -