TY - JOUR ID - 16999 TI - T-cell Tolerance Following Bacterial Glutamic Acid Decarboxylase (GAD) Feeding in Streptozotocin-induced Diabetes JO - Iranian Journal of Immunology JA - IJI LA - en SN - 1735-1383 AU - Fani, Fereshteh AU - kamali-Sarvestani, Eskandar AU - Yazdanparast, Razieh AU - Monabati, Ahmad AU - Rafiei, Shahnaz AD - Department of Immunology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran AD - Department of Immunology and AD - Institute of Biochemistry and Biophysics, Tehran University, Tehran, Iran, and AD - Department of Pathology, Faculty of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran Y1 - 2006 PY - 2006 VL - 3 IS - 4 SP - 169 EP - 175 KW - Escherichia coli KW - Glutamic Acid Decarboxylase KW - Streptozotocin KW - Oral Tolerance DO - N2 - Background: Autoimmune type 1 diabetes mellitus is caused by T-cell mediated immune destruction of the insulin-producing β-cell in pancreatic islets of Langerhans. Specificity of the auto-antibodies and of the auto-reactive T-cells has been investigated, in which several auto-antigens were proposed. Objective: To determine whether glutamic acid decarboxylase (GAD) feeding would induce oral tolerance of   either T-cell or B-cell compartment in streptozotocin (STZ) diabetic rats. Methods: Rats in the experimental group were fed 2 mg/kg of GAD (extracted from Escherichia coli ) 14 days before intra-peritoneal injections of streptozotocin (30 mg/kg body weight for 5 consecutive days). Two control groups were considered: diabetic control group, which underwent STZ injections without receiving GAD, and normal control group. Systemic response was compared between the three groups. T-cells response was assessed by a proliferation assay of spleen cells and those of the B-cells by enzyme-linked immunosorbent assay (ELISA) for anti-GAD specific antibodies in serum. Results: Compared with the diabetic control group, a significant reduction was observed only in the proliferative response of spleen cells, but not in the level of anti-GAD antibody. Conclusion: GAD feeding induces systemic T-cell tolerance in STZ-induced diabetes. UR - https://iji.sums.ac.ir/article_16999.html L1 - https://iji.sums.ac.ir/article_16999_e5df23476e3a8b4f4c85eacd769c4e34.pdf ER -