TY - JOUR ID - 17037 TI - An In Vitro Assay to Evaluate the Immunomodulatory Effects of Unrestricted Somatic Stem Cells JO - Iranian Journal of Immunology JA - IJI LA - en SN - 1735-1383 AU - Ebrahimi, Hasan AU - Soleimani, Masoud AU - Kazemi Arabadadi, Mohammad AU - Ajmadbeigi, Naser AU - Hassanshahi, Gholamhossein AU - Farjadfar, Akbar AU - Kennedy, Derek AD - Department of Molecular Medicine, Pasteur Institute of Iran, Tehran, Iran AD - Department of Hematology, Tarbiat Modares University, Tehran, Iran AD - Department of Microbiology, Hematology and Immunology, Faculty of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran AD - Stem Cell Department, Stem Cell Research Center, Tehran, Iran AD - Department of Molecular Medicine, Pasteur Institute of Iran, Tehran, Iran AD - School of Biomolecular and Biomedical Sciences, Eskitis Institute for Cell and Molecular Therapies, Griffith University Nathan, Queensland, Australia Y1 - 2010 PY - 2010 VL - 7 IS - 1 SP - 30 EP - 38 KW - Mesanchymal Stem Cells KW - Mixed Lymphocyte Reaction KW - Transplantation DO - N2 - Background: Unrestricted somatic stem cells (USSC) are cord blood stem cells that have been considered as candidates for the regulation of immune responses. Therefore, potential exists for their use in the suppression of immune response after transplantation surgery. Objective: The aim of this study was evaluation of the effect of USSC on mixed lymphocyte reaction (MLR) as a model for graft rejection. Methods: USSC and mesanchymal stem cells (MSC) were isolated and cultured from cord blood and bone morrow, respectively. The immunophenotypes of USSC and MSC were evaluated by flow cytometery and USSC and MSC were co-cultured with peripheral blood lympho-cytes (PBL) in an MLR to evaluate the immunomodulatory effect of these cells as a percentage of the control response. Results: Current study demonstrated that prolifera-tion of lymphocytes in the MLR was decreased after treatment with USSC, in a similar fashion to that seen with MSC. Conclusion: It can be concluded that USSC have simi-lar regulatory effects as MSC on the MLR, which can be used as an indicator for poten-tial organ rejection after transplantation. Therefore, the immunregulatory effect of these cells could be used in the clinic during organ transplantation and in the management of autoimmunity. UR - https://iji.sums.ac.ir/article_17037.html L1 - https://iji.sums.ac.ir/article_17037_9614818d509d024d51f557c42538fac4.pdf ER -