TY - JOUR ID - 17164 TI - Effects of T-2 Toxin on Cytokine Produc-tion by Mice Peritoneal Macrophages and Lymph Node T-Cells JO - Iranian Journal of Immunology JA - IJI LA - en SN - 1735-1383 AU - Ahmadi, Kazem AU - Riazipour, Majid AD - Research Center of Molecular Biology, Baqiyatallah University of Medical Sciences, Tehran, Iran Y1 - 2008 PY - 2008 VL - 5 IS - 3 SP - 177 EP - 180 KW - T-2 toxin KW - IL-1β KW - IL-12 KW - TNF-α KW - IL-2 KW - IFN-γ DO - N2 - Background: T-2 toxin is a mycotoxin of type A trichothecenes produced by several fungal genera such as Fusarium species. Mycotoxins can affect both cell mediated and humoral immune compartments. Objective: The purpose of this study was to investi-gate the effect of T-2 toxin on cytokine production by mouse peritoneal macrophages and lymph node T cells. Methods: Mouse peritoneal macrophages and lymph node T cells were isolated and treated with different concentrations of T-2 toxin and incubated at 370C and 5% CO2 in air for 48 hours. Cell free media were removed and used for cy-tokine assay by an ELISA method. Results: T-2 toxin significantly reduced IL-1β re-lease in a concentration dependent manner (p<0.005, p<0.001). Interleukin-12 and TNF-α production were significantly increased in response to 0.001ng/ml, 0.01ng/ml and 0.1ng/ml of T-2 toxin (p<0.001). However, T-2 toxin at higher concentrations rang-ing from 1ng/ml to 100ng/ml, reduced both IL-12 (p<0.001) and TNF-α production (p<0.005, p<0.05). The effects of T-2 toxin on lymph node T cells showed that IL-4 and IL-10 release was decreased in a concentration dependent manner (all with p<0.01). T-2 toxin at concentrations between 1ng/ml and 100ng/ml reduced the release of both IL-2 and IFN-γ (p<0.05, p<0.001). Conclusion: The results suggest that T-2 toxin at low concentrations can highly induce secretion of IL-12, TNF-α, IFN-γ and IL-2 and it may be used as a positive immunomodulator in the human model. UR - https://iji.sums.ac.ir/article_17164.html L1 - https://iji.sums.ac.ir/article_17164_4f5f79a1238234ab8c6de41c45a20107.pdf ER -