TY - JOUR ID - 17169 TI - Null Allele Frequencies at HLA-G Locus in Iranian Healthy Subjects JO - Iranian Journal of Immunology JA - IJI LA - en SN - 1735-1383 AU - Rahimi, Roghayeh AU - Zavaran Hosseini, Ahmad AU - Yari, Fatemeh AD - Tarbiat Modares University, School of Medical Sciences AD - Research Center, Iranian Blood Transfusion Organization, Tehran, Iran Y1 - 2008 PY - 2008 VL - 5 IS - 4 SP - 207 EP - 211 KW - Frequency KW - HLA-G*0105N antigen KW - Iran KW - RFLPs DO - N2 - Background: HLA-G gene contains 15 alleles including a null allele, HLA-G*0105N. Previous studies have shown that HLA-G*0105N does not encode the complete HLA-G1 or HLA-G5 isoforms but encodes a functional HLA-G protein with the ability to in-hibit NK cell cytolysis. Thus, although the biological functions of HLA-G1 and HLA-G5 proteins are abrogated, other isoforms such as HLA-G2 can replace their roles. Stud-ies on the null allele of HLA-G gene could be useful in understanding the genetic vari-ants of HLA-G alleles in ethnic groups. Objective: The goal of this research was to de-termine the frequency of HLA-G*0105N null allele in Iranian healthy subjects. Meth-ods: The frequency of HLA-G*0105N null allele was evaluated in Iranian healthy sub-jects by PCR-RFLP method. Genomic DNA was isolated from the whole blood of 100 randomly selected, healthy, unrelated Iranian individuals using salting-out technique followed by PCR amplification of the exon 3 of HLA-G gene. PCR products were di-gested with PpUM-1 and the resulted fragments were analyzed using gel electrophore-sis. Results: In this study the restriction enzyme digestion confirmed homozygous HLA-G*0105N null allele for 9 % of the population. Furthermore obtained results indi-cated that the total frequency of HLA-G*0105N null allele was 20 % in the studied population of Iran. Conclusion: The final data analysis showed that the total frequency of this allele in Iranian people was higher than other ethnic groups that have been stud-ied so far. UR - https://iji.sums.ac.ir/article_17169.html L1 - https://iji.sums.ac.ir/article_17169_3ec26db0c327dcd23463357af63dd097.pdf ER -