TY - JOUR ID - 39276 TI - CD8+ T Lymphocyte Subsets in Bladder Tumor Draining Lymph Nodes JO - Iranian Journal of Immunology JA - IJI LA - en SN - 1735-1383 AU - Faghih, Zahra AU - Sadat Shobeiri, Saeideh AU - Ariafar, Ali AU - Sarkarian, Mohsen AU - Zeighami, Shahryar AU - Nazari, Nazanin AU - Abbasi-Sarvak, Saeed AU - Erfani, Nasrollah AD - Cancer Immunology Group, Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran AD - Urology-Oncology Research Center,Shiraz University of Medical Sciences, Shiraz, Iran AD - Department of Urology, Shiraz University of Medical Sciences, Shiraz, Iran AD - Urology-Oncology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran AD - School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran Y1 - 2016 PY - 2016 VL - 13 IS - 4 SP - 237 EP - 248 KW - Bladder Cancer KW - Lymph Node KW - CD8+ lymphocytes KW - Tc1 KW - Tc2 KW - Tc17 DO - N2 - Background: Cytotoxic CD8+ T cells, as essential parts of the adaptive immune system, play pivotal roles in anti-tumor immune responses. It is well documented that cytokine expression profiles and activation status of these cells during anti-tumor immune responses affect the outcome of host-tumor interaction. Objective: To investigate the percentages of CD8+ lymphocytes and their subsets in tumor draining lymph nodes of patients with bladder cancer. Methods: Forty-five patients with bladder cancer, candidate for radical cystectomy, were recruited. Mononuclear cells were isolated from draining lymph nodes using Ficoll-Hypaque gradient centrifugation, and were activated by PMA/Ionomycin in the presence of Golgi inhibitors. The cells were then permeabilized and stained with appropriate flourochrome conjugated antibodies against CD3, CD8, IFN-γ, IL-17 and IL-4 molecules. Data were collected on a fourcolor flow cytometer and analyzed by CellQuestPro software. Results: Despite no difference in the frequency of IL-17 producing CD8+ (Tc17) lymphocytes, the mean expression of IL-17 in this subset was significantly elevated in high-grade patients (p=0.011). The percentage of double positive IFN-γ/IL-17 CD8+ lymphocytes was also significantly increased in node positive patients compared to node negative ones (p=0.046). Our results also demonstrated that the percentage of IFN-γ producing CD8+ (Tc1) lymphocytes was significantly increased in the patients with higher histological grade compared to those with lower ones (p=0.038). Conclusion: IFN-γ and IL-17 producing CD8+ T cells may increase in advanced stages of bladder cancer, but their correlation with tumor prognosis remains to be investigated. UR - https://iji.sums.ac.ir/article_39276.html L1 - https://iji.sums.ac.ir/article_39276_b4a0d75e62ba8bf71d6ed4a8d8473928.pdf ER -