TY - JOUR ID - 39308 TI - Antitumor Response to a Codon-Optimized HPV-16 E7/HSP70 Fusion Antigen DNA Vaccine JO - Iranian Journal of Immunology JA - IJI LA - en SN - 1735-1383 AU - Soleimanjahi, Hoorieh AU - Razavinikoo, Hadi AU - Fotouhi, Fatemeh AU - Ardebili, Abdollah AD - Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran AD - Laboratory Science Research Center, Golestan University of Medical Sciences, Gorgan, Iran AD - Influenza Research Lab, Department of Virology, Pasteur Institute of Iran, Tehran, Iran Y1 - 2017 PY - 2017 VL - 14 IS - 3 SP - 180 EP - 191 DO - N2 - Background: Vaccines based on virus-like particles are effective against Human Papilloma Virus (HPV) infection; however, they have not shown a therapeutic effect against HPV-associated diseases. New immunotherapy strategies based on immune responses against tumor antigens can positively affect the clearance of HPV-associated lesions. Objective: To generate two therapeutic fusion DNA vaccines (optimizedE7/mouseHSP70 and wildE7/mouseHSP70) to induce antitumor specific responses in mice models. Methods: Mice were immunized with recombinant DNA vaccines. The splenocytes of immunized mice were collected and lactate dehydrogenase and IFN-γ productions were measured after three injections in order to evaluate cytotoxic T lymphocytes (CTLs) activity. MTT assay was carried out for lymphocyte stimulation. Results: The fusion DNA vaccines, specifically uE7-HSP70, elicited varying levels of IFN-γ and CTLs responses compared to the control group (P<0.05). Furthermore, antitumor response and tumor size reduction in fusion DNA vaccines groups were significantly higher than in the negative control group (P<0.05). Conclusion: It is concluded that our fusion DNA vaccines considerably enhanced specific cellular responses against HPV tumor model. In addition, optimized E7 showed a notable immunogenicity and inhibitory effect on the reduction of tumor size. UR - https://iji.sums.ac.ir/article_39308.html L1 - https://iji.sums.ac.ir/article_39308_06a6513856f9ff2890487ea2d125671d.pdf ER -