TY - JOUR ID - 39337 TI - Expression of IGF-1, IL-27 and IL-35 Receptors in Adjuvant Induced Rheumatoid Arthritis Model JO - Iranian Journal of Immunology JA - IJI LA - en SN - 1735-1383 AU - Abdi, Elham AU - Najafipour, Hamid AU - Joukar, Siyavash AU - Dabiri, Shahriar AU - Esmaeli-Mahani, Saeed AU - Abbasloo, Elham AU - Houshmandi, Nasrin AU - Afsharipour, Abbas AD - Physiology Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran AD - Cardiovascular Research Center, Institute of Basic and Clinical Physiology Sciences and Department of Physiology and Pharmacology, Kerman University of Medical Sciences, Kerman, Iran AD - Pathology and Stem Cell Research Center, Kerman University of Medical Sciences, Kerman, Iran AD - Department of Biology, Shahid Bahonar University, Kerman University of Medical Sciences, Kerman, Iran AD - Endocrinology and Metabolism Research Center, Kerman University of Medical Sciences, Kerman, Iran AD - Gastroenterology and Hepathology Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences, Kerman, Iran Y1 - 2018 PY - 2018 VL - 15 IS - 1 SP - 14 EP - 27 DO - N2 - Background: IGF-1 and certain other cytokines have been shown to exert inflammatory/anti-inflammatory roles in chronic joint diseases. Objective: To assess the effect of IGF-1, IL-27 and IL-35, their interaction and their receptor expression in a rheumatoid arthritis model. Methods: Freund’s adjuvant-induced chronic joint inflammation was operated on 160 male rats. Animals were divided into histopathology and receptor expression groups, each composed of 10 subgroups including; control, vehicle, IGF-1, IL-27, IL-35, their antagonists, IGF-1+IL-27 antagonist and IGF-1+IL-35 antagonist. After two weeks, vehicle or agonist/antagonists were injected into the joint space every other day until day 28 where joint histopathology was performed. The expression of IGF-1, IL-27 and IL-35 receptors were assessed by western blot analysis. Results: IGF-1 did not show pro- or anti- inflammatory functions; endogenous IL-27 and IL-35, on the other hand, exerted inflammatory effects. IL-27 and IL-35 antagonists exerted the highest anti-inflammatory effects. The total inflammation scores were 0.55 ± 0.06, 4.63 ± 0.40, 3.63 ± 0.60, 2.50 ± 0.38 and 1.63 ± 0.40 regarding control, vehicle, IGF-1 Ant., IL-27 Ant. and IL-35Ant., respectively. IGF-1 receptor expression was reduced in chronic joint inflammation and all three antagonists augmented the IGF-1 receptor expression. IL-27 and IL-35 receptors were up-regulated by chronic joint inflammation. Conclusion: Overall, the results demonstrated the pro-inflammatory role of endogenous IL-27 and IL-35 along with the over expression of their receptors in chronic joint inflammation. IL-27 and IL-35 antagonists exerted the most anti-inflammatory effects and increased IGF-1 receptor expression. These two antagonists may be potential agents for new treatment strategies in chronic joint inflammatory diseases. UR - https://iji.sums.ac.ir/article_39337.html L1 - https://iji.sums.ac.ir/article_39337_48604e5d63802c2db190b7ac43b401e5.pdf ER -