TY - JOUR ID - 45973 TI - Importance of L. Infantum H2B Recombinant Antigen for Serodiagnosis of Visceral Leishmaniasis JO - Iranian Journal of Immunology JA - IJI LA - en SN - 1735-1383 AU - Rezaei, Zahra AU - Pouladfar, Gholamreza AU - Ramezani, Amin AU - Mostafavi-Pour, Zohreh AU - Abbasian, Amin AU - Sarkari, Bahador AU - Pourabbas, Bahman AD - Professor Alborzi Clinical Microbiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran AD - Institute for Cancer Research, Shiraz University of Medical Sciences, Shiraz, Iran AD - Department of Biochemistry, Recombinant Protein Laboratory, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran AD - Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran Y1 - 2019 PY - 2019 VL - 16 IS - 4 SP - 311 EP - 320 KW - Iran KW - Human KW - Recombinant H2B Antigen KW - Serological Diagnosis KW - visceral leishmaniasis DO - 10.22034/iji.2019.80282 N2 - Background: Visceral leishmaniasis (VL) can lead to death in more than 95% of cases if left untreated. Accurate and early diagnosis has an important role in reducing mortality rate of this disease. Objective: To express recombinant H2B antigen from an Iranian isolate of Leishmania Infantum and evaluate its efficacy in the diagnosis of VL. Methods: The recombinant H2B antigen was produced in a prokaryotic system, and its efficacy for VL diagnosis was evaluated by ELISA. The serum samples from 80 VL patients, 100 individuals from endemic and non-endemic regions of VL, and 58 non-VL patients were collected. VL cases were confirmed based on the clinical sign, positive IFAT (>64), real time PCR, and response to treatment. Results: The H2B gene sequence of the Iranian L. infantum isolate had about 4% diversity in comparison with the H2B gene of the L. infantum counterpart. ELISA, using the produced H2B recombinant antigen, showed sensitivity of 71.25% (95% CI: 60.05%-80.82%) and specificity of 69.62% (95% CI: 61.81%-76.68%) regarding VL diagnosis. Conclusion: Recombinant H2B antigen expressed in the prokaryotic system had suboptimal performance for the serological diagnosis of VL. It seems that the production and expression of recombinant H2B antigen in a eukaryotic system may enhance the performance of this antigen in the diagnosis of VL in Iran. UR - https://iji.sums.ac.ir/article_45973.html L1 - https://iji.sums.ac.ir/article_45973_9919fa48c030c6c40a905df94df1d18a.pdf ER -