Shiraz Institute for Cancer ResearchIranian Journal of Immunology1735-13833220060601Expression of CXC Chemokines Gro/KC and SDF-1a in Rat H4 Hepatoma Cells in Response to Different Stimuli546016957ENGholamhosseinHassanshahiImmunology and Hematology Departments, Medical School, Rafsanjan University of Medical Sciences,
Rafsanjan, IranMohammadKazemi ArababadiImmunology and Hematology Departments, Medical School, Rafsanjan University of Medical Sciences,
Rafsanjan, Iranoooo-0002-7705-431xAlanJames DicksonBiochemistry Department, Faculty of Life Sciences, Manchester University, Manchester, UKJournal Article20160806<b>Background</b>: It is now well established that several environmental stress factors cause activation of p38 MAP kinase and JNK in various cell types to produce chemokines. <br/><b>Objective</b>: To investigate the expression of CXC chemokines Gro/KC and SDF- 1a in rat's H4 hepatoma cells in response to heat shock, hyperosmolarity and oxidative stress. <br/><b>Methods</b>: Hepatoma cells were maintained in MEM medium. Cells were subjected to different stresses [(H2O2 0.15% (w/v), manitol and NaCl (160 mM) and heat shock (42 °C for 20 minutes)]. Cells were harvested and RNA was extracted, purified and the CXC chemokine Gro/KC and SDF-1a expression was analysed by RT-PCR. cDNA was separated by gel electrophoresis on a 1% (w/v) agarose gel and visualized under a UV transilluminator. <br/><b>Results</b>: There was detectable but low expression of both SDF-1a and Gro/KC in H4 hepatoma cells. Heat shock failed to induce expression of SDF-1a and Gro/KC in H4 hepatoma cells of rat. Hyperosmolarity also did not stimulate SDF-1a and Gro/KC expression. In this study we have also shown that oxidative stress did not induce expression of SDF-1a and Gro/KC. Overall, although detection is possible but regulatory responses were not observed in H4 hepatoma cells. <br/><b>Conclusion</b>: Several known injurious conditions cause recruitment of macrophages, neutrophils and other immune cells to the liver. Immune cells are recruited to the hepatic vasculature following local liver injury and subsequent chemokine production. Our results demonstrated that failure to produce chemokines by hepatoma cells may be a way to escape from mechanism of immune surveillance.Shiraz Institute for Cancer ResearchIranian Journal of Immunology1735-13833220060601Determination of Soluble HER-2/neu (sHER-2/neu) in Iranian Patients with Lung Cancer616516960ENSeyyed Mohammad AliGhayumiDepartment of Internal MedicineKambizAghasadeghiDepartment of Internal MedicineMehrnooshDorouchiDepartment of ImmunologyAbbasGhaderiDepartment of ImmunologyShiraz Institute for Cancer Research,
Shiraz University of Medical Sciences, Shiraz, Iran0000-0003-0849-3375Journal Article20160806<b>Background</b>: The HER-2/neu gene is located on chromosome 17q21 and encodes a 185-kDa transmembrane glycoprotein with tyrosine kinase activity reported to be released in soluble form in various malignancies. <br/><b>Objective</b>: To evaluate the clinical significance of soluble Her-2/neu as a diagnostic marker in lung cancer. <br/><b>Methods</b>: Serum levels of soluble HER-2/neu were measured in 43 patients with lung cancer and 42 age and sex matched controls by an enzyme immunoassay method. <br/><b>Results</b>: Mean serum level of soluble Her-2/neu in cancer patients was 6.07±10.37 ng/ml which was significantly higher than the control group (P < 0.05). Cigarette smoking had no effect on the level of soluble HER-2/neu. A cut off value of 6.1ng/ml revealed a high specificity (95%) for diagnosis of lung cancer, but a very low sensitivity (14%). <br/><b>Conclusion</b>: The results of this study show an increased level of soluble HER-2/neu in the sera of lung cancer patients with a high specificity but low sensitivity for diagnosis of lung cancers.Shiraz Institute for Cancer ResearchIranian Journal of Immunology1735-13833220060601Anti-streptokinase Antibody Detection before and Immediately after Streptokinase Therapy in Patients with Myocardial Infarction666916961ENHasanShemiraniFaculty of Medicine, Isfahan University of Medical Sciences, Isfahan, IranAbbasRezaeiFaculty of Medicine, Isfahan University of Medical Sciences, Isfahan, IranJournal Article20160806<b>Background</b>: Myocardial infarction (MI) is one of the most common and serious diseases resulting from coronary artery occlusion and major reduction in blood flow. Streptokinase as a thrombolytic is considered the first and most important therapeutic intervention for reperfusion following MI in most countries including Iran. Our previous study showed that, the prevalence of high antibody titers against streptokinase was 13.5% in the studied population from Iran, which was 2.5 times more common than data from other studies. <br/><b>Objective</b>: To evaluate anti-streptokinase antibody titers before and immediately after streptokinase administration and its relation to reperfusion therapy success rates. <br/><b>Methods</b>: A total of 200 patients with acute MI was selected. Antibodies against streptokinase were measured before and 2 days after administration of streptokinase. Before streptokinase administration and every hour after streptokinase administration, for 3 consecutive hours, an ECG was taken from each participant and changes were evaluated in relation to antibody levels. <br/><b>Results</b>: Out of 200 patients, 42 (21%) had high levels of antibody titer. Out of these 42 patients, 13 (6.5%) still had measurable levels of anti-streptokinase antibody after streptokinase administration. <br/><b>Conclusion</b>: Our results show the ability of the antistreptokinase antibody to neutralize the effects of streptokinase.Shiraz Institute for Cancer ResearchIranian Journal of Immunology1735-13833220060601Evaluation of Cellular Immune Response against Purified Antigen 85 in Patients with Tuberculosis707716965ENIrajNikokarMicrobiology and Immunology of Infectious Disease Laboratory, Guilan University of Medical
SciencesManouchehrMakvandiDepartment of Microbiology, Ahwaz University of Medical Sciences0000-0002-7488-6479Mohammad JavadKajbafDepartment of Microbiology, Ahwaz University of Medical SciencesAhmadFarajzadehDepartment of Microbiology, Ahwaz University of Medical SciencesMehdiMirsaeidiDepartment of Tuberculosis,
National Research Institute of Tuberculosis and Lung Diseases, Shahid Beheshti University of
Medical SciencesEskandarKamali-SarvestaniDepartment of Immunology andAutoimmune Diseases Research Center, Shiraz
University of Medical Sciences,AliMostafaieDepartment of Microbiology, Kermanshah University of Medical SciencesKrisHuygenPasteur Institute of Brussels, BelgiumJournal Article20160806<b>Background</b>: Tuberculosis (TB) remains an important health problem throughout the world. Despite its significance in public health, mechanisms of protective immunity against Mycobacyerium Tuberculosis in humans have not yet been understood. <br/><b>Objective</b>: To evaluate cell mediated immune response against purified Ag 85, PPD and Phytohemagglutinin (PHA) in patients with tuberculosis and healthy tuberculin positive and negative individuals. <br/><b>Methods</b>: Thirty patients with tuberculosis and 60 healthy tuberculin skin test positive and negative volunteers were participated in this study. Cell mediated immunity was assessed by measuring [³H]-thymidine uptake and detection of IFN-γ in the culture supernatant using commercial ELISA test. <br/><b>Results</b>: In the present study, we showed that IFN- γ production and cell proliferation response to Ag 85 were significantly higher in tuberculin positive than tuberculin negative individuals (P<0.01). Among tuberculous patients, IFN-γ production and cell proliferative responses to Ag 85 was significantly lower in contrast to healthy tuberculin positive individuals (P<0.01). In addition, IFN- γ response in patients with cavitary tuberculosis was lower than patients without cavitation (P<0.05). <br/><b>Conclusion</b>: Based on the higher cell mediated immune responses to Ag 85 in healthy tuberculin positive volunteers compared to patients (especially with advanced disease), purified Ag 85 can be used as a sensitive marker for analysis of immune responses in tuberculosis.Shiraz Institute for Cancer ResearchIranian Journal of Immunology1735-13833220060601Protective Role of Antigens from Peritoneal Exudates of Infected Mice against Toxoplasmosis788516970ENAhmadDaryaniDepartment of Parasitology and Mycology, Medical School, Mazandaran University of Medical
Sciences, Sari, IranAhmadZavaran HosseiniDepartment of Immunology and Department of Parasitology, Tarbiat Modarres
University of Medical Sciences, Tehran, IranMehdiSharifDepartment of Parasitology and Mycology, Medical School, Mazandaran University of Medical
Sciences, Sari, IranAbdolhoseeinDalimiDepartment of Immunology and Department of Parasitology, Tarbiat Modarres
University of Medical Sciences, Tehran, IranMohammad HosseinDehghanDepartment of Basic Sciences, Medical School, Ardabil
University of Medical Sciences, Ardabil, IranHajarZiaeiDepartment of Parasitology and Mycology, Medical School, Mazandaran University of Medical
Sciences, Sari, IranJournal Article20160806<b>Background</b>: Toxoplasma gondii is an obligate intracellular parasite that infects all mammalian cells. Several antigens such as excreted/secreted antigens have been identified as a potential vaccine candidate. <br/><b>Objective</b>: To determine how excreted/secreted antigens from peritoneal exudates of infected mice (mESA) stimulate cell-mediated immune responses and induce protective immunity against toxoplasmosis in the murine model. <br/><b>Methods</b>: The supernatants produced from the peritoneal fluids, were fractionated by precipitation in ammonium sulphate solution (30-80% saturated). For induction of cell-mediated immune responses, delayed type hypersensitivity was measured, in injected footpad. Response to purified antigen was measured by lymphocyte proliferation assay. Nitric oxide was measured by Griess method. For immunization, Balb/c mice were immunized 2 times with mESA, mESA-40% and Toxoplasma Lysate Antigen (TLA). The virulent RH strain of Toxoplasma gondii was used for challenging. <br/><b>Results</b>: The pattern of lymphocyte responsiveness was dependent on the antigen employed. In sensitized mice, those received mESA-40% displayed higher lymphocyte response than mice stimulated by mESA (p<0.05). The highest amounts of nitric oxide were observed in macrophages, which received mESA-40% and mESA (p<0.05). Mice immunized with mESA-40% survived longer than those immunized with mESA and other antigens (p<0.05). <br/><b>Conclusion</b>: As fraction 40% (mESA-40%) showed a good result in induction of cellmediated responses in the murine model, the purification and isolation of the mESA 40% is highly recommended for future study.Shiraz Institute for Cancer ResearchIranian Journal of Immunology1735-13833220060601Aqueous Levels of Anti-Helicobacter Pylori IgG Antibody in Patients with Primary Open Angle and Pseudoexfoliation Glaucoma869016971ENMohammad RezaRazeghinejadDepartments of Ophthalmology andEskandarKamali-SarvestaniImmunologyAutoimmune Diseases Research Center, Shiraz
University of Medical Sciences, Shiraz, IranMohsenFarvardinDepartments of Ophthalmology andArashPourhabibiDepartments of Ophthalmology andJournal Article20160806<b>Background</b>: Glaucoma is a progressive optic neuropathy and is one of the leading causes of blindness worldwide. Different factors have been contributed in the pathogenesis of glaucoma including H. pylori infection. <br/><b>Objective</b>: To determine the levels of anti-H. pylori IgG antibody in the aqueous humor of patients with pseudoexfoliation and primary open angle glaucoma, in comparison with age and sex matched cataract patients. <br/><b>Methods</b>: This study was conducted on 41 cases of glaucoma (21 with pseudoexfoliation and 20 with primary open angle glaucoma) and 39 cases of cataract as control group. Aqueous humor was aspirated at the beginning of glaucoma or phacoemulsification cataract surgery in glaucoma and cataract patients, respectively. Anti-H. pylori IgG concentration was measured by means of an enzyme-linked immunosorbent assay. <br/><b>Results</b>: The aqueous levels of anti-H. pylori IgG in primary open angle glaucoma (0.44±0.64 U/ml) had no significant difference with cataract (0.24±0.52U/ml) and pseudoexfoliation glaucoma group (0.63±0.71U/ml) (P=0.18 and 0.44, respectively). However, the concentration of this antibody was higher in the aqueous humor of pseudoexfoliation glaucoma patients compared to the control group (p=0.03). <br/><b>Conclusion</b>: The results of this study did not support a relation between H. pylori infection and primary open angle glaucoma. The elevated concentration of anti-H. pylori IgG in pseudoexfoliation glaucoma compared to cataract patients may be due to the breakdown of blood-aqueous-barrier.Shiraz Institute for Cancer ResearchIranian Journal of Immunology1735-13833220060601The Role of Immune System in Idiopathic Anterior Uveitis919416973ENMansourRahimiDepartment of Ophthalmology, Shiraz University of Medical Sciences, Shiraz, IranMortezaNajafiDepartment of Ophthalmology, Shiraz University of Medical Sciences, Shiraz, IranJournal Article20160806<b>Background</b>: Idiopathic anterior uveitis is an anterior segment inflammation in which a detailed medical history, general and ocular physical examination is not associated with any defined clinical syndrome. Alterations in immune system parameters have been reported in patients with idiopathic posterior uveitis; however no data on the role of immune system in idiopathic anterior uveitis has yet been reported. In this study the immune system function in patients with idiopathic anterior uveitis was evaluated. <br/><b>Objective</b>: To evaluate the immune system function in patients with idiopathic non-infectious anterior uveitis. <br/><b>Methods</b>: 51 patients with anterior uveitis, 32 women (62.7%) and 19 men (37.3%), participated in this study. Intensity of intraocular inflammation was scored according to standard uveitis grading system. In all cases, serum levels of immunoglobulins A, G, M and E, C3 and C4 complement components, and autoantibodies against ds-DNA and ACLA, were measured using ELISA method. <br/><b>Results</b>: 49 patients out of 51 (96%) showed altered serum levels of immunological parameters, compared with normal values. Changes in serum immunoglobulin concentration were present in 44 patients, with increased IgA levels being the most common. Serum values of C3 and C4 complement proteins were also increased in 29 subjects. ds-DNA autoantibody was positive in 15 and equivocal in 19 cases. ACLA was positive and equivocal in 3 and 9 patients, respectively. <br/><b>Conclusion</b>: Immune abnormalities found in serum of 49 patients with idiopathic anterior uveitis may play a role in the pathogenesis of this disorder.Shiraz Institute for Cancer ResearchIranian Journal of Immunology1735-13833220060601Typing of HLA Class I by Polymerase Chain Reaction-Sequence Specific Oligonucleotide Primer (PCR-SSOP) Technique in Iranian Cord Blood Donors959816974ENAndishehGhashghaie MansourHematology, Oncology, and Bone Marrow Transplantation Research Center, Tehran University of
Medical sciences, Tehran, IranSeyyed HamidollahGhaffariHematology, Oncology, and Bone Marrow Transplantation Research Center, Tehran University of
Medical sciences, Tehran, IranKamranAli-moghadamHematology, Oncology, and Bone Marrow Transplantation Research Center, Tehran University of
Medical sciences, Tehran, IranArdeshirGhavamzadehHematology, Oncology, and Bone Marrow Transplantation Research Center, Tehran University of
Medical sciences, Tehran, IranJournal Article20160806<strong>Background</strong>: HLA compatibility between transplant donor and recipient is one of the major determinants of transplant outcome. <br/><strong>Objective</strong>: To determine HLA class I by PCR- Sequence-Specific Oligonucleotide Probe (PCR-SSOP) in cord blood donors. <br/><strong>Methods</strong>: Genomic DNA of 142 cord blood samples registered at the Cord Blood Bank of Iran at Hematology, Oncology, and Bone Marrow Transplantation Research Center, was prepared and HLA class I was determined by the PCR-SSOP. <br/><strong>Results</strong>: A total of 284 HLA-A alleles was identified of which A*02 and A*24 were the most common. Among 284 HLA-B and HLA-C alleles, B*35, B*51, Cw*4 and Cw*12 were the most frequent alleles in the studied population. <br/><strong>Conclusion</strong>: Amplification of HLA loci with PCR-SSOP has proved to be a reliable method for HLA-A, -B and -C genotyping.