Mohammad Momeni; Mohammad Reza Mirzaei; Nahid Zainodini; Gholamhossein Hassanshahi; Mohammad Kazemi Arababadi
Volume 10, Issue 2 , June 2013, , Pages 103-109
Abstract
Background: Absent in Melanoma 2 (AIM2) is an intracellular microbial dsDNA sensor which plays an important role in production of proinflammatory cytokines through Apoptosis associated Speck-like protein containing a Caspase activation and recruitment domain (ASC) and Caspase-1. Micro-RNAs (miRNAs) play ...
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Background: Absent in Melanoma 2 (AIM2) is an intracellular microbial dsDNA sensor which plays an important role in production of proinflammatory cytokines through Apoptosis associated Speck-like protein containing a Caspase activation and recruitment domain (ASC) and Caspase-1. Micro-RNAs (miRNAs) play important roles in regulation of immune related genes. However, there is little information regarding the effects of miRNAs on the AIM2 and ASC expression. Objective: To determine the mRNA levels of AIM2 and ASC in Jurkat cell line following introducing miRNA-143 (MiR-143). Methods: MiR-143, a scrambled sequence and PBS were introduced separately, to the Jurkat cell lines and the mRNA levels of AIM2 and ASC were examined in parallel with beta-actin and GAPDH (as housekeeping genes) using Real-Time PCR technique. Results: The mRNA levels of AIM2 and ASC were significantly increased in the MiR-143 transfected Jurkat cells when compared to the scrambled sequence or PBS treated cells. Conclusions: MiR-143 can lead to increased expression of AIM2 and ASC mRNAs. Considering the significance of AIM2 and ASC in DNA sensing and inflammosome formation, it can be considered as a therapeutic agent for the treatment of chronic infectious diseases, especially viral infections.
Nahid Zainodini; Gholamhossein Hassanshahi; Mohammad Kazemi Arababadi; Hossein Khorramdelazad; Afshin Mirzaei
Volume 10, Issue 1 , March 2013, , Pages 40-46
Abstract
Background: Alopecia Areata (AA) is a non-scarring, autoimmune disorder which causes hair loss. Inflammatory reactions are involved in hair loss of the scalp and/or body. The involvement of chemokine receptors in the pathogenesis of AA is well defined among which, CXCL1 acts on neutrophils and CXCL9, ...
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Background: Alopecia Areata (AA) is a non-scarring, autoimmune disorder which causes hair loss. Inflammatory reactions are involved in hair loss of the scalp and/or body. The involvement of chemokine receptors in the pathogenesis of AA is well defined among which, CXCL1 acts on neutrophils and CXCL9, CXCL10 and CXCL12 and serve as T lymphocytes recruiters. Objective: To study the serum levels of ELR+ and ELR- CXCL1, CXCL9, CXCL10 and CXCL12 in the patients suffering from AA and healthy controls. Methods: The study population of consisted of 30 patients suffering from AA and 30 healthy controls. Serum concentrations of CXCL1, CXCL9, CXCL10 and CXCL12 were measured using enzymelinked immunosorbent assay (ELISA). Results: Current results showed that AA patients had significantly elevated serum levels of CXCL9 and CXCL10 in comparison to controls (p<0.001). These results also demonstrated that serum levels of CXCL1 and CXCL12 were significantly decreased in AA patients compared to control (p<0.001). Conclusion: CXCL9 and CXCL10 are elevated in the AA patients and may be involved in the recruitment of T lymphocytes to the inflamed tissues. Moreover, due to the significant role played by these chemokines in angiogenesis/angiostatis phenomenon they could be considered as useful biomarkers in AA diagnosis and therapy.
