Iranian Journal of Immunology

Analysis of Serum Cytokine Levels in Larynx Squamous Cell Carcinoma and Dysplasia Patients

Document Type : Original Article

Authors

Mete Eyigor 1
Hulya Eyigor 2
Ustun Osma 2
MustafaDeniz Yilmaz 2
Nuray Erin 3
Omer Tarik Selcuk 2
Cem Sezer 4
Meral Gultekin 1
Sadi Koksoy 1

1 Akdeniz University Medical Faculty, Department of Medical Microbiology

2 Department of ENT Head and Neck Surgery, Antalya Education and Research Hospital

3 Akdeniz University Medical Faculty, Department of Medical Pharmacology

4 Department of Pathology, Antalya Education and Research Hospital, Antalya, Turkey

https://doi.org/10.22034/iji.2014.16786
Abstract
Background: Although the imbalance of cytokines in Head and Neck Squamous Cell Carcinoma (HNSCC) is well known, there is scarce data regarding its occurrence during dysplasia, before the malignant transformation.
Objective: To determine whether laryngeal dysplasia patients show a different cytokine profile than patients with cancer and healthy controls.
Methods: Seventeen newly diagnosed, untreated larynx squamous cell carcinoma (SCC) and six laryngeal dysplasia patients as well as 22 healthy controls were analyzed for circulating cytokines. A flowcytometry Th1/Th2 cytokine array kit was used to quantitatively measure Interleukin-2 (IL-2), IL-4, IL-6, IL-10, Tumor Necrosis Factor-α (TNF-α) and Interferon-γ (IFN-γ) levels. Additionally, IL-8 levels were determined through ELISA.
Results: IL-6, IL-8 and IL-10 were determined to be statistically increased in SCC patients (p<0.05). IL-8 and IL-10 levels were also higher in SCC patients than dysplasia patients (p<0.05). Additionally, IL-6 and IL-10 were all found to be markedly increased in dysplasia patients compared with controls (p<0.05).
Conclusion: Our results demonstrate an imbalance of IL-6 and IL-10 not only in HNSCC but also in laryngeal dysplasia.

Keywords

Bead Array
Cytokine
Dysplasia
HNSCC
Larynxm Carcinoma
Iranian Journal of Immunology
Volume 11, Issue 4
Autumn 2014
Pages 259-268

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