Document Type: Original Article

Authors

1 Department of Parasitology and Mycology

2 Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran

Abstract

Background: Different methods have been used for characterization of Leishmania promastigotes. Monoclonal antibodies are useful in characterization of   Leishmania spp . both amastogotes and promastigotes.
Objective:
Comparing the characterization of   Leishmania spp. promastigotes with immunoperoxidase test (Avidin-Biotin) techniques and an indirect immunofluorescent assay (IFA).  
Methods:
Application of specific monoclonal antibodies for characterization of different   Leishmania species. Immunoperoxidase tests (Avidin-Biotin) and indirect immunofluorescent assay (IFA) were employed for characterization of different   Leishmnia promastigotes from culture. Five monoclonal antibodies including LXXVIII-2E5- A8 (D2) specific for   L. donovani:L. infantum , IS2-2B4 (A11) specific for L. tropica, XCIV-H2- AB (T10) specific for   L. tropica, XLVI-5B8- B3 (T1) specific for L. major, and T7 reactive to both   L. major and L. tropica as well as an anti GP63 mAb were used.
Results:
The best result was obtained with the dilution of 1:50 for both mAb and conjugate. One hundred percent sensitivity and specificity was achieved for characterization of Leishmania promastogotes with both methods.
Conclusion:
As immunoperoxidase method needs less equipments compared to IFA technique, it would be a preferred method for characterization of promastigotes.

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