Hamideh Mesali; Abolghasem Ajami; Hadi Hussein-Nattaj; Alireza Rafiei; Zeinab Rajabian; Hossein Asgarian-Omran; Vahid Hosseini; Tarang Taghvaei; Mohsen Tehrani
Volume 13, Issue 3 , September 2016, , Pages 167-177
Abstract
Background: Regulatory T Cells (Tregs) and Myeloid-Derived Suppressor Cells (MDSCs) are two main regulatory cells modulating the immune responses in inflammation and cancer. Objective: To investigate and compare Tregs and MDSCs in peptic ulcer and gastric cancer. Methods: Patients with dyspepsia were ...
Read More
Background: Regulatory T Cells (Tregs) and Myeloid-Derived Suppressor Cells (MDSCs) are two main regulatory cells modulating the immune responses in inflammation and cancer. Objective: To investigate and compare Tregs and MDSCs in peptic ulcer and gastric cancer. Methods: Patients with dyspepsia were selected and divided into three groups of non-ulcer dyspepsia (NUD, n=22), peptic ulcer disease (PUD, n=25), and gastric cancer (GC, n=27) according to their endoscopic and histopathological examinations. Helicobacter pylori infection was diagnosed by rapid urease test and histopathology. The number of peripheral blood CD4+CD25+FoxP3+Tregs and CD14+HLA-DR- MDSCs were determined in all patients, by flow cytometry. The number of FoxP3+ regulatory T cells was also determined by immunohistochemistry (IHC). Results: The percentage of peripheral blood Treg cells in both PUD )0.81 ± 0.39, p<0.001) and GC groups )0.98 ± 0.65, p<0.001) were significantly higher than in NUD group (0.46 ± 0.10). These results were also confirmed by IHC. A significantly higher percentage of MDSCs in patients with PUD )0.73 ± 0.19, p<0.001) and GC )0.73 ± 0.16, p<0.001) was also observed when compared to NUD group )0.46 ± 0.16). There was no difference in the percentages of these two cell types between the PUD and GC groups. The percentages of Tregs and MDSCs in patients with PUD and GC were not significantly correlated. Conclusions: Both Tregs and MDSCs showed higher frequencies in PUD and GC. These results suggest that immune-modulation by the Tregs and MDSCs may play a role in the pathogenesis of PUD and GC.
Abolghasem Ajami; Amir Esmailnejad Moghaddam; Hasan Motamed
Volume 1, Issue 3 , December 2004, , Pages 177-182
Abstract
Background: Antifertility effect of naturally occuring antisperm antibody (ASA) in infertile couples and studies on experimental immunization of various animals with sperm antigens represents ASA as immunocontraceptive target. Despite extensive research on the effects of different factors on sperm immunogenecity ...
Read More
Background: Antifertility effect of naturally occuring antisperm antibody (ASA) in infertile couples and studies on experimental immunization of various animals with sperm antigens represents ASA as immunocontraceptive target. Despite extensive research on the effects of different factors on sperm immunogenecity and ASA production variable result have been reported. Objective: To study whole sperm immunization in mice. Methods: In an experimental study, whole mice sperm with different adjuvant i.e. complete Freund’s adjuvant (CFA), incomplete Freund’s adjuvant (ICFA), and cholera toxin subunit- β (CTS-β) were administrated to mice intramuscularly (IM), subcutaneously (SC), intranasally (IN), intra-peritoneally (IP), intrarectally (IR), intravaginally (IVA) and orally. Control groups were inoculated with phosphate buffer saline (PBS) plus corresponding adjuvant. Immunization was carried out on days 0, 7, 14, 28 and ASA titers were detected by indirect immunofluorescence (IFA) technique in sera and vaginal washes of all groups. The IP group was further excluded from the study due to high mortality rate. The results were compared between control and experimental groups by Mann Whitney and Fisher exact tests. Results: The number of positive mice for ASA in IM, SC, IN experimental and control groups were significantly different (P = 0.01, P = 0.01, P = 0.04, respectively). However, there were no significant differences between IR, IVA, and oral experimental and control groups. No differences were observed between ASA in vaginal washing of all groups. Due to high mortality in IP group it was excluded from the study. Conclusion: It can be concluded that the whole sperm antigen can induce immune response in female mice by IM, SC, IN but not IAV, IR and oral administration routes.