QingXiao Su; LiJun Jiang; Jia Chai; ZhiYan Dou; ZanHua Rong; Xue Zhao; Bo Yu; YuXue Wang; XinLiang Wang
Abstract
Background: Purpuric nephritis is the most common secondary glomerular disease in childhood. Its prevalence in children has been steadily rising in recent years. Objective: To explore the characteristics and pathogenesis of changes in peripheral blood lymphocyte subsets and immune function in children ...
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Background: Purpuric nephritis is the most common secondary glomerular disease in childhood. Its prevalence in children has been steadily rising in recent years. Objective: To explore the characteristics and pathogenesis of changes in peripheral blood lymphocyte subsets and immune function in children with Henoch-Schonlein purpura nephritis. Methods: The study included 104 children with Henoch-Schonlein purpura, divided into nephritis (HSPN) group (68 cases) and non-nephritis (NHSPN) group (36 cases), and 15 normal children. The rate-scatter turbidimetric method was utilized to determine the immunoglobulins IgA, IgG, IgM, C3 and C4, and the flow cytometry technique was employed to detect the levels of lymphocyte subsets including CD3+, CD4+, CD8+, CD4+/CD8+, CD19+, NK, etc. Results: Compared with the control group, the CD3+, CD4+, CD8+ and NK cell levels of peripheral blood mononuclear cells significantly decreased (p <0.05), and the CD19+ level significantly elevated (p <0.05) in the HSPN group and the NHSPN group whereas the HSPN group had a more significant change than the NHSPN group (p <0.05). Compared with the control group, the serum immunoglobulin IgA and IgG of the HSPN group and the NHSPN group significantly increased, and the IgM, C3, and C4 significantly decreased (p <0.05); while the HSPN group had a more significant change than the NHSPN group (p <0.05). Conclusion: Immune dysfunction in children with HSPN is specifically manifested as low cellular immune function, which leads to increased secretion of inflammatory mediators, activates B cells, and further increases the secretion of immunoglobulins, leading to the occurrence of small vasculitis.
Atefeh Kamallou; Mahbobeh Haji Abdolbaghi; Minoo Mohraz; Mernaz Rasolinejad; Ehsan Karbasi; Bita Ansaripour; Samaneh Soltani; Arezou Rezaei; Neda Khalili; Aliakbar Amirzargar
Volume 11, Issue 4 , December 2014, , Pages 221-232
Abstract
Background: Lymphocyte subsets enumeration is considered prominent in the management of primary and acquired immunodeficiency disorders. Because of local variations due to race, age, gender, and environmental conditions on lymphocyte subsets, and to improve the accuracy of interpretation of laboratory ...
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Background: Lymphocyte subsets enumeration is considered prominent in the management of primary and acquired immunodeficiency disorders. Because of local variations due to race, age, gender, and environmental conditions on lymphocyte subsets, and to improve the accuracy of interpretation of laboratory findings, reference intervals must be determined in every population. Objective: To establish a normal reference range for CD3+ , CD4+ , CD8+ , CD19+ and CD56+ lymphocytes in a healthy Iranian adult population using flowcytometry. Method: Blood samples were collected from 221 HIV seronegative individuals, including 112 females and 109 males, with ages ranging from 20 to 40 years old. The percentage of lymphocytes expressing either of CD3, CD4, CD8, CD19 and CD56 surface markers were determined by flowcytometry assay. Result: Total mean percentage and absolute count of lymphocyte subsets were as follows: CD3+ : 70.90 ± 7.54%, 1800.87 ± 471.09 cells/µl; CD4+ : 41.04 ± 7.86%, 1039.99 ± 338.02 cells/µl; CD8+ : 31.11 ± 6.60%, 783.95 ± 234.87 cells/µl; CD19+ : 12.77 ± 4.56%, 328.37 ± 153.17 cells/µl; CD56+ : 15.53 ± 6.34%, 388.62 ± 176.17 cells/µl, respectively. The ratio of CD4+ /CD8+ lymphocytes for the studied population was 1.39 ± 0.48. Significant differences were observed between male and female subjects indicating that the average percentage of CD3+ cells (p=0.017) and CD4+ T cells (p=0.003) were higher in the female population, whereas the average percentage of CD19+ cells (p=0.02) tended to be higher among males. However, investigations on the CD56+ NK cell and CD8+ T cell sub-populations did not show any statistical differences between the two genders. In comparison with reports of other populations, we were confronted with different results. Conclusion: Establishing reference values of lymphocyte subsets for each population is helpful in achieving standard criteria for the prognosis of HIV infection. Therefore, normal ranges established by this survey can be used as a reference for decisions made in clinical practice.
Ghasem Mosayebi; Shaban Ali Alizadeh; Ali Alasti; Alireza Amouzandeh Nobaveh; Ali Ghazavi; Mahsa Okhovat; Mohammad Rafiei
Volume 10, Issue 4 , December 2013, , Pages 216-228
Abstract
Background: The appendix is considered as part of the gut-associated lymphoid tissue; however, lymphocyte subsets in this tissue are not fully defined. Objective: To investigate and compare the function and phenotype of lymphocyte subsets in peripheral blood and appendix of patients with normal and inflamed ...
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Background: The appendix is considered as part of the gut-associated lymphoid tissue; however, lymphocyte subsets in this tissue are not fully defined. Objective: To investigate and compare the function and phenotype of lymphocyte subsets in peripheral blood and appendix of patients with normal and inflamed appendix tissues. Methods: Peripheral blood samples and appendiceal mononuclear cells were obtained from 81 patients (mean age; 23 ± 10.5 years), clinically suspected of having appendicitis. The phenotypic characteristics of lymphocyte subsets in peripheral blood (before and 48-72 hrs after appendectomy) and in appendix tissue were analyzed by three color-flow cytometry. The proliferative response of mononuclear cells was assessed by MTT method. Results: The frequency of CD19+DR+, HLA-DR+ and CD19+ cells in the appendix tissue were significantly higher than that of the peripheral blood in all the groups (p<0.001). The percentage of CD19+ cells and HLA-DR+CD19+ cells significantly decreased after appendectomy in the peripheral blood of the patients with acute appendicitis (p=0.047 and p=0.03, respectively). CD19 and HLA-DR plus CD19 had better diagnostic efficiency compared with T cell markers (area under the ROC curve [AUC]= 0.76 and 0.73, respectively). Conclusion: These results indicate a significant difference in CD19+ and HLA-DR+ lymphocytes between peripheral blood and the appendix tissue.
