Shiquan Xu; Qing Liu; Jie Wang; Zeyun Zhang; Ying Wang; Li Li; Tao Zhang; Yu Fan
Abstract
Background: Previous research has identified several potential biomarkers associated with pathological tumor (pT) staging in prostate cancer (PCa) patients. Among these biomarkers, CX3CR1 is notable for its connection to the immune microenvironment.Objective: To further investigate the significance of ...
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Background: Previous research has identified several potential biomarkers associated with pathological tumor (pT) staging in prostate cancer (PCa) patients. Among these biomarkers, CX3CR1 is notable for its connection to the immune microenvironment.Objective: To further investigate the significance of CX3CR1 as a key biomarker for predicting pT staging and PCa progression.Methods: Prostate cancer tissue samples were analyzed using quantitative reverse transcription polymerase chain reaction (qRT-PCR) and immunohistochemical staining. The diagnostic performance of CX3CR1 was evaluated using receiver operating characteristic (ROC) curves, while Kaplan–Meier survival analysis was conducted to determine overall survival (OS) rates.Results: A significant decrease in CX3CR1 expression was observed in PCa tissues compared to adjacent normal tissues, with the lowest levels detected in pT3 tumors. CX3CR1 expression showed a negative correlation with preoperative prostate-specific antigen (PSA) levels, lymph node staging (N stage), Gleason score, and overall survival (OS). Additionally, CX3CR1 levels were associated with the polarization of infiltrating CD4+ T cells in PCa patients.Conclusion: CX3CR1, as a biomarker associated with pT staging, plays a role in predicting PCa prognosis, potentially by modulating the immune microenvironment.
Ruiping Li; Wei Song; Donglan Mei
Abstract
Background: Severe chronic obstructive pulmonary disease (COPD) patients with pulmonary infections face higher morbidity and mortality.Objective: To investigate mononuclear cell membrane CD14 as a prognostic marker for their outcome.Methods: A total of 311 participants were included: 122 in the coinfection ...
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Background: Severe chronic obstructive pulmonary disease (COPD) patients with pulmonary infections face higher morbidity and mortality.Objective: To investigate mononuclear cell membrane CD14 as a prognostic marker for their outcome.Methods: A total of 311 participants were included: 122 in the coinfection group, 127 in the severe COPD group, and 62 in the control group. The patients in the coinfection group were categorized into survival (n=106) and death (n=16) groups based on hospitalization prognosis. The CD14%, CD14MFI, and CD14IND values were compared between the groups. Death risk factors were assessed by COPD grading, FEV1% pred, FEV1/FVC, CD14%, CD14MFI, and CD14IND. Correlations between CD14 parameters and mortality, COPD grade, FEV1%pred, and FEV1/FVC were analyzed. The critical value for CD14IND to predict patient death was determined and survival rates were compared between the high and the low-risk groups.Results: CD14% values were significantly lower in the COPD and co-infection groups than in the control groups (p<0.05). The survival group showed a steady increase in mCD14 expression, while the death group showed fluctuating low levels. Low value of CD14% was identified as a risk factor for death and correlated with mortality and COPD severity (p<0.001). CD14IND≤74.36 predicted death with 91.22% sensitivity and 95.51% specificity. The high-risk group had a significantly lower 30-day survival rate (68.42%) compared with the low-risk group (95.24%) (log-rank χ2=10.067, p=0.002).Conclusion: The CD14 parameters of mononuclear cell membranes prove to be promising markers for predicting prognosis and death in severe COPD patients with lung infection.
Zhanming Sha; Panpan Zhang
Abstract
Background: The relationship between genetic polymorphism and postoperative pain and the prognosis of patients with hepatocellular carcinoma (HCC) undergoing hepatectomy is not fully understood. Objective: To examine whether lncRNA-GAS5 and its promoter region rs145204276 polymorphism can predict ...
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Background: The relationship between genetic polymorphism and postoperative pain and the prognosis of patients with hepatocellular carcinoma (HCC) undergoing hepatectomy is not fully understood. Objective: To examine whether lncRNA-GAS5 and its promoter region rs145204276 polymorphism can predict postoperative pain and prognosis of the patients with HCC undergoing hepatectomy. Methods: Seventy patients with HCC undergoing hepatectomy were enrolled. The lncRNA-GAS5 levels in CD4+ T cells from peripheral blood mononuclear cells (PBMC-CD4+ T cells) and tumor tissues were measured by qRT-PCR. Genotyping analysis of rs145204276 was performed using the TaqMan platform. PBMC-CD4+ T cells were isolated and the cytokine levels in helper T (Th) cells were determined by flow cytometry. Patients with Ins/Ins genotype carrying the rs145204276 polymorphism were allocated into the Ins group, and others were allocated into the Del group. Results: The lncRNA-GAS5 level decreased significantly in PBMC-CD4+ T cells and tumor tissues compared with the healthy controls and corresponding adjacent non-tumor tissues. The patients with Del/Del genotype showed significantly higher lncRNA-GAS5 expression in PBMC-CD4+ T cells, lower postoperative pain scores, and better overall survival. LncRNA-GAS5 expression in PBMC-CD4+ T cells was negatively associated with IL-6, IL-17, and the RORγT/CD3 ratio (an indicator of TH17 polarization). Conclusion: LncRNA-GAS5 expression and its promoter region rs145204276 polymorphism are prognostic biomarkers that can predict postoperative pain of patients with HCC undergoing hepatectomy.
adel ebrahimpour; Ahamadreza Mirbolook; mohammadali okhovatpour; mohammadreza sajadi; kamyar makvandi; mohamad sadegh mousavi; sepehr saghari; mehrdad sadighi
Abstract
Background: Interleukin 6 (IL-6) functions as both a pro-inflammatory cytokine and an anti-inflammatory cytokine. Objective: To evaluate the levels of IL-6 in patients with multiple organ dysfunction syndrome (MODS). Methods: Level of IL-6 was assessed and recorded for 14 days subsequent to the injury ...
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Background: Interleukin 6 (IL-6) functions as both a pro-inflammatory cytokine and an anti-inflammatory cytokine. Objective: To evaluate the levels of IL-6 in patients with multiple organ dysfunction syndrome (MODS). Methods: Level of IL-6 was assessed and recorded for 14 days subsequent to the injury in 161 multiple trauma patients. MODS were diagnosed using Marshal Score. Injury Severity Scoring (ISS) was measured for all patients. Results: The results of this study indicated that there was a significant relationship between the level of IL-6 and ISS on the post trauma days number one and two (P=0.0001). The high level of IL-6 on the post trauma day number 2 was associated with high mortality rate. Conclusion: Our study suggests the second day as the golden time for measuring the serum levels of IL-6. These findings warn us to take more health care actions in patients with higher serum levels of IL-6 on the second day.
