Document Type : Original Article
Authors
- Mohammad Javad Fattahi 1
- Abdul Mohammad Pezeshki 2
- Maryam Emad 3
- Mohammad Hosein Lohrasb 4
- Azra Shamseddin 5
- Abbas Ghaderi 1, 2
- Mehrnoosh Doroudchi 1, 2
1 Department of Immunology
2 Shiraz Institute for Cancer Research
3 Department of Dermatology, Shiraz University of Medical Sciences, Shiraz, Iran
4 Department of Immunology and
5 Dermatology, Fasa Medical School, Fasa, Iran
Abstract
Background: Vitiligo is an acquired skin disorder that selectively destroys melanocytes in epidermis with an unknown etiology.
Objective: To investigate the exon 1 A49G polymorphism of cytotoxic T lymphocyte antigen-4 (ctla-4) gene in vitiligo patients.
Methods: The A49G polymorphism was detected by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method in 101 patients and 208 normal healthy age/ethnicity matched individuals.
Results: The frequencies of heterozygote genotypes in patients and controls were found to be 42 (41.6%) of 101 and 85 (40.9%) of 208, respectively. The frequencies of homozygote A and G genotypes were 49 (48.5%) and 10 (9.9%) in 101 patients, whereas, these frequencies in 208 control individuals were 103 (49.5%) and 20 (9.6%), respectively. There was no significant difference between the genotype (P = 0.98) and allele (P = 0.86) frequencies of A49G polymorphism in patients and normal healthy individuals.
Conclusion: Our results indicate that in contrast to several immune mediated disorders, there is no association between ctla-4 A49G gene polymorphism and vitiligo.
Keywords
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