Iranian Journal of Immunology

Comparison of Neutrophil Apoptosis α-Defensins and Calprotectin in Children with and without Severe Early Childhood Caries

Document Type : Original Article

Authors

Lida Toomarian 1
Mandana Sattari 2
Nazanin Hashemi 1
Nikoo Tadayon 1
Alireza Akbarzadeh Baghban 3

1 Department of Pediatric Dentistry, Dental School

2 Department of Immunology, Shahid Beheshti University of Medical Sciences

3 Department of Biostatics, Shahid Beheshti University of Medical Sciences, Tehran, Iran

https://doi.org/10.22034/iji.2011.16883
Abstract
Background: The infectious nature of severe early-childhood caries (S-ECC) points to the possible participation of immunologic host responses including neutrophils and their antimicrobial products.
Objectives: The aim of this study was to determine the neutrophil apoptosis, α-defensins (HNP1-3) and calprotectin levels in the saliva of preschool children and the association with S-ECC.
Methods: Oral examinations were performed on 87 children aged 3-5 years and non stimulated whole saliva samples were collected. Thirty of these subjects were considered S-ECC children, 30 with moderate caries (MC) and 27 were caries free (CF). To detect apoptosis, cell staining was done with Annexin-V-Fluos and propidium iodide, and they were analyzed by fluorescent microscopy. The concentration of α-defensins and calprotectin were assessed using ELISA.
Results: There were no statistical differences between groups considering the HNP1-3 or calprotectin salivary levels (p=0.06 and p=0.23, respectively). The HNP1-3 and calprotectin levels were negatively correlated and the correlation was significant in MC group (p=0.03). Lower levels of apoptotic neutrophils were obtained from CF subjects as compared with S-ECC children (p=0.03).
Conclusions: Our findings establish that apoptotic mechanisms could be implicated in the immunity responses associated with S-ECC. We cannot yet

Keywords

Severe Early Childhood Caries
apoptosis
α-Defensins
Calprotectin
Neutrophil
Iranian Journal of Immunology
Volume 8, Issue 1
Winter 2011
Pages 11-19

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