Background: HLA compatibility between transplant donor and recipient is one of the major determinants of transplant outcome. Objective: To determine HLA class I by PCR- Sequence-Specific Oligonucleotide Probe (PCR-SSOP) in cord blood donors. Methods: Genomic DNA of 142 cord blood samples registered at the Cord Blood Bank of Iran at Hematology, Oncology, and Bone Marrow Transplantation Research Center, was prepared and HLA class I was determined by the PCR-SSOP. Results: A total of 284 HLA-A alleles was identified of which A*02 and A*24 were the most common. Among 284 HLA-B and HLA-C alleles, B*35, B*51, Cw*4 and Cw*12 were the most frequent alleles in the studied population. Conclusion: Amplification of HLA loci with PCR-SSOP has proved to be a reliable method for HLA-A, -B and -C genotyping.
Ghashghaie Mansour, A., Ghaffari, S. H., Ali-moghadam, K. & Ghavamzadeh, A. (2006). Typing of HLA Class I by Polymerase Chain Reaction-Sequence Specific Oligonucleotide Primer (PCR-SSOP) Technique in Iranian Cord Blood Donors. Iranian Journal of Immunology, 3(2), 95–98. https://doi.org/10.22034/iji.2006.16974
MLA
Ghashghaie Mansour, A., Ghaffari, S. H., Ali-moghadam, K., & Ghavamzadeh, A. "Typing of HLA Class I by Polymerase Chain Reaction-Sequence Specific Oligonucleotide Primer (PCR-SSOP) Technique in Iranian Cord Blood Donors", Iranian Journal of Immunology, 3, 2, 2006, 95–98. doi: 10.22034/iji.2006.16974
HARVARD
Ghashghaie Mansour, A., Ghaffari, S. H., Ali-moghadam, K., & Ghavamzadeh, A. (2006). 'Typing of HLA Class I by Polymerase Chain Reaction-Sequence Specific Oligonucleotide Primer (PCR-SSOP) Technique in Iranian Cord Blood Donors', Iranian Journal of Immunology, 3(2), pp. 95–98. doi: 10.22034/iji.2006.16974
CHICAGO
Ghashghaie Mansour, A., Ghaffari, S. H., Ali-moghadam, K. & Ghavamzadeh, A., "Typing of HLA Class I by Polymerase Chain Reaction-Sequence Specific Oligonucleotide Primer (PCR-SSOP) Technique in Iranian Cord Blood Donors." Iranian Journal of Immunology, 3 2 (2006): 95–98, doi: 10.22034/iji.2006.16974
VANCOUVER
Ghashghaie Mansour A., Ghaffari S. H., Ali-moghadam K., Ghavamzadeh A. Typing of HLA Class I by Polymerase Chain Reaction-Sequence Specific Oligonucleotide Primer (PCR-SSOP) Technique in Iranian Cord Blood Donors. Iran.J.Immunol. 2006;3(2):95–98. doi: 10.22034/iji.2006.16974
