Iranian Journal of Immunology

Characterization of Immune Responses Induced by Combined Clade-A HIV-1 Recombinant Adenovectors in Mice

Document Type : Original Article

Authors

Sayeed Bayanolhagh 1
Mahtab Alinezhad 2
Kooroosh Kamali 3
Maryam Foroughi 4
Hamid Reza Khorram Khorshid 5
Minoo Mohraz 4
Fereidoun Mahboudi 6
Ali Akbar Pourfathollah 1

1 Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University

2 Research and Development Complex, Pasteur Institute of Iran

3 Department of Epidemiology and Biostatics, Tehran University of Medical Sciences

4 Iranian Research Center for HIV/AIDS, Tehran University of Medical Sciences

5 Genetic Research Centre, University of Social Welfare and Rehabilitation Sciences

6 Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran

https://doi.org/10.22034/iji.2010.17053
Abstract
Background: Numerous evidences indicate that in some HIV-1 positive patients, the humoral and cellular immune responses are induced against HIV-1 proteins and this is inversely related to the progress of infection.
Objective: The aim of this study was the evaluation of the Adenovectors containing HIV genes in induction of immune responses in mice.
Methods: The HIV-1 genes including gag p24, rev, nef and exon-1 of tat were amplified from HIV-1 RNA (clade-A). The cDNA of each gene was cloned into a transfer vector. The transfer vector was then co-transformed into E. coli strain BJ5183 together with pAdenovector ΔE1/E3. The recombinant adenoviral construct was transfected into QBI-293A cells. Recombinant viruses were purified and titrated on 293 cell plates. Expression of transgenes was evaluated using western blotting. Then 1012 viral particles were injected into 15 groups of 5 mice and all patterns of combination of these 4 HIV-1 genes were evaluated. After 2 weeks, humoral and cellular immune responses were evaluated using ELISA, cell proliferation and ELISpot (IL-2, IL-4 and IFN-γ) assays, consecutively.
Results: It was demonstrated that each gene was expressed. The response targets were mostly toward Th1, though several Th2 responses were also observed. Single injection in our study induced a good cellular response but the humoral responses were not as strong as the cellular ones.
Conclusion: Considering and comparing all results and evaluating the various possible interactions revealed that simultaneous injection of tat and gag has enhanced the humoral and cellular responses.

Keywords

Adenovector
Cellular Responses
HIV Vaccine
Humoral Immune Responses
Iranian Journal of Immunology
Volume 7, Issue 3
Summer 2010
Pages 162-176

Home

Guide for Authors

Submit Manuscript

Guide for Reviewers

Contact Us