Background: Dendritic cells (DC) are a key regulator of the immune response, and interferon- beta (IFN-β) is considered an immunomodulatory molecule for DC. Objective: The purpose of this study was to evaluate the ability of IFN-β treated DC to induce cytokine secretion by CD4+ T cells. Methods: Dendritic cells were generated from blood monocytes with granulocyte-monocyte colony-stimulating factor and interleukin-4 with or without IFN-β. We analyzed the production of CD4+ T helper cytokines (IL-17, IFN- γ and IL-10) in the supernatant of the dendritic cell-T cell co- cultures by ELISA. We also studied the effects of HLA-G and costimulatory molecules on immature and mature DC. Results: IFN-γ and IL-17 decreased significantly in the presence of HLA-Gbearing DC compared to control cultures (p<0.05). Conclusion: Using the mixed leukocyte reaction, we found that DC treated with IFN-β mediated the inhibition of T cell activation via cytokine production. We conclude that this is important for preventing overactivation of the immune system.
Abediankenari, S., Shaker, D., Abedian, F. & Mirabi, A. (2009). The Effect of Beta Interferon on Dendritic Cells and Cytokine Synthesis by CD4+ T Cells. Iranian Journal of Immunology, 6(2), 61–66. https://doi.org/10.22034/iji.2009.17075
MLA
Abediankenari, S., Shaker, D., Abedian, F., & Mirabi, A. "The Effect of Beta Interferon on Dendritic Cells and Cytokine Synthesis by CD4+ T Cells", Iranian Journal of Immunology, 6, 2, 2009, 61–66. doi: 10.22034/iji.2009.17075
HARVARD
Abediankenari, S., Shaker, D., Abedian, F., & Mirabi, A. (2009). 'The Effect of Beta Interferon on Dendritic Cells and Cytokine Synthesis by CD4+ T Cells', Iranian Journal of Immunology, 6(2), pp. 61–66. doi: 10.22034/iji.2009.17075
CHICAGO
Abediankenari, S., Shaker, D., Abedian, F. & Mirabi, A., "The Effect of Beta Interferon on Dendritic Cells and Cytokine Synthesis by CD4+ T Cells." Iranian Journal of Immunology, 6 2 (2009): 61–66, doi: 10.22034/iji.2009.17075
VANCOUVER
Abediankenari S., Shaker D., Abedian F., Mirabi A. The Effect of Beta Interferon on Dendritic Cells and Cytokine Synthesis by CD4+ T Cells. Iran.J.Immunol. 2009;6(2):61–66. doi: 10.22034/iji.2009.17075
