Iranian Journal of Immunology

Production and Characterization of a Monoclonal Antibody against an Antigen on the Surface of Non-Small Cell Carcinoma of the Lung

Document Type : Original Article

Authors

Sedigheh Sharifzadeh 1, 2
Helmout Modjtahedi 3
Mahmood Jedi Tehrani 4
Abbas Ghaderi 2, 5

1 Department of Laboratory Science, Paramedical School

2 Department of Immunology, Shiraz University of Medical Sciences, Shiraz, Iran

3 Postgraduate Medical School, University of Surrey, Guildford, United–Kingdom

4 Avecina Institute, Tehran, Iran

5 Shiraz Institute for Cancer Research, Shiraz, Iran

https://doi.org/10.22034/iji.2007.17199
Abstract
Background: Lung carcinoma is a multiple type cancer comprising of small cell and non-small cell carcinomas (NSCLC). For therapeutic and diagnostic purposes, serum monoclonal antibodies have been produced against lung cancer.
Objective: To charac-terize a murine monoclonal antibody (ME3D11) reactive with human NSCLC.
Methods: A murine monoclonal antibody (ME3D11) reactive with human NSCLC was selected after immunization of BALB/c mice with a human large cell carcinoma with neuroen-docrine differentiation, and was tested by immunofloursence staining and Western blot analysis.
Results: Our study showed that the antigen recognized by ME3D11 antibody was a cell surface antigen of 170kDa. This antigen is expressed on the cell surface of all NSCLC and a few carcinoma cell lines. In contrast, this antigen is neither expressed on the cell surface of human sarcoma, nor on the hematopoietic and normal cell lines. This anti-body had no effect on spontaneous proliferation of Mehr-80 cell line in vitro.
Conclusion: High degree of binding of this monoclonal antibody to NSCLC and some other carci-noma cells warrants further studies on its potential use in diagnosis and therapy of can-cer by conjugation to drugs, toxins or radionuclides.

Keywords

NSCLC
ME3D11
Mehr-80
Iranian Journal of Immunology
Volume 4, Issue 4
Autumn 2007
Pages 206-214

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