Background: Mesenchymal stem cells (MSCs) and transforming growth factor-β1 (TGF-β1) molecules are well known for their immunomodulatory properties and their function in tissue regeneration and remodeling. Objectives: To evaluate the interaction of TGF-β1 engineered MSCs with T cells and dendritic cells (DCs) and their modulatory effect on the immune response. Methods: MSCs and DCs were generated from bone marrow of Balb/c mice and T cells were generated from mice lymph nodes. TGF-β1 expressing lentiviruses were used for MSCs transduction, and then these engineered MSCs were co-cultured with T cells and DCs. T cells proliferation and cytokines release and also DCs maturation, TNF-α release, and stimulation of allogeneic T cells were evaluated. Results: T cells proliferation and IFN-γ release were suppressed by TGF-β1/MSCs while IL-4 secretion was enhanced. Co-cultured DCs with TGF-β1/MSCs showed reduced expression of CD40, CD86, and MHC II and also lower level of TNF-α secretion. Co-cultured DCs could also induce lower levels of allogeneic T cells proliferation and IFN-γ release in comparison to control DCs. Conclusion: Engineered TGF-β1/MSC cells showed collaborative immune suppressive functions between TGF-β1 and MSCs to modulate T cells and DCs immune responses. We therefore suggest that TGF-β1/MSC cells could provide a promising tool for treatment of clinical conditions such as organ transplantation, GVHD, and autoimmune disorders.
Daneshmandi, S., Karimi, M. H. & Pourfathollah, A. A. (2017). TGF-Β1 Transduced Mesenchymal Stem Cells Have Profound Modulatory Effects on DCs and T Cells. Iranian Journal of Immunology, 14(1), 13–23. https://doi.org/10.22034/iji.2017.39285
MLA
Daneshmandi, S., Karimi, M. H., & Pourfathollah, A. A. "TGF-Β1 Transduced Mesenchymal Stem Cells Have Profound Modulatory Effects on DCs and T Cells", Iranian Journal of Immunology, 14, 1, 2017, 13–23. doi: 10.22034/iji.2017.39285
HARVARD
Daneshmandi, S., Karimi, M. H., & Pourfathollah, A. A. (2017). 'TGF-Β1 Transduced Mesenchymal Stem Cells Have Profound Modulatory Effects on DCs and T Cells', Iranian Journal of Immunology, 14(1), pp. 13–23. doi: 10.22034/iji.2017.39285
CHICAGO
Daneshmandi, S., Karimi, M. H. & Pourfathollah, A. A., "TGF-Β1 Transduced Mesenchymal Stem Cells Have Profound Modulatory Effects on DCs and T Cells." Iranian Journal of Immunology, 14 1 (2017): 13–23, doi: 10.22034/iji.2017.39285
VANCOUVER
Daneshmandi S., Karimi M. H., Pourfathollah A. A. TGF-Β1 Transduced Mesenchymal Stem Cells Have Profound Modulatory Effects on DCs and T Cells. Iran.J.Immunol. 2017;14(1):13–23. doi: 10.22034/iji.2017.39285
